Shalini Anandan1, Dhiviya Prabaa Muthuirulandi Sethuvel2, Revathi Gajendiren2, Valsan Philip Verghese3, Kamini Walia4, Balaji Veeraraghavan5. 1. MD, Department of Clinical Microbiology, Asha building, Christian Medical College, Ida scudder road, Vellore, 632004, India. 2. MSc, Department of Clinical Microbiology, Asha building, Christian Medical College, Ida scudder road, Vellore, 632004, India. 3. MD, Department of Child Health, ISSCC building, Christian Medical College, Ida scudder road, Vellore, 632004, India. 4. PhD, Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, Ansari Nagar, New Delhi, 110029, India. 5. PhD, Department of Clinical Microbiology, Asha building, Christian Medical College, Ida scudder road, Vellore, 632004, India.
Abstract
BACKGROUND: Shigella species are an important cause of acute diarrheal disease worldwide. This study describes the prevalence of Shigella spp. serotypes and their resistance profile in Vellore, South India from 2014 to 2015. METHODS: From 2014 to 2015, 338 Shigella strains were isolated from stool samples at Christian Medical College, Vellore, India. Identification and serotyping was carried out using standard protocols. Antimicrobial susceptibility testing was done against commonly used antibiotics. Multidrug resistance was detected in 157 isolates. A subset of 73 isolates was randomly characterized further for acquired antimicrobial resistance genes in this study. RESULTS: The resistance profile of the study isolates varied by species and year. S. sonnei isolates were 100% resistant to all tested antibiotics in 2014, whereas in 2015, resistance was found for AMP-NAL-TAX-SXT-FIX. The resistance phenotypes among S. flexneri isolates for the year 2014 and 2015 were AMP-SXT-NAL-NOR-FIX-TAX and AMP-NAL-SXT-TAX-NOR-FIX respectively. Screening for antimicrobial resistance genes in S. flexneri found dhfr1A, sulII, blaOXA, blaTEM, blaCTX-M-1,qnrB, qnrS and AmpC genes while S. sonnei were found to have only dhfr1A, sulII, blaCTX-M-1 and qnrS genes respectively. Antimicrobial resistance genes were predominantly seen in AMP-SXT-NAL and AMP-SXT-NAL-NOR resistance phenotypes. CONCLUSION: Shigella prevalence of 4.8% to 4.6% was documented between the years 2014 to 2015 in this study. We show evidence that resistance to commonly used antibiotics continues to increase among Shigella spp. in South India. The presence of qnrS and blaCTX-M-15 in the study isolates further indicates the threat of spreading resistance to quinolones and third-generation cephalosporins.
BACKGROUND: Shigella species are an important cause of acute diarrheal disease worldwide. This study describes the prevalence of Shigella spp. serotypes and their resistance profile in Vellore, South India from 2014 to 2015. METHODS: From 2014 to 2015, 338 Shigella strains were isolated from stool samples at Christian Medical College, Vellore, India. Identification and serotyping was carried out using standard protocols. Antimicrobial susceptibility testing was done against commonly used antibiotics. Multidrug resistance was detected in 157 isolates. A subset of 73 isolates was randomly characterized further for acquired antimicrobial resistance genes in this study. RESULTS: The resistance profile of the study isolates varied by species and year. S. sonnei isolates were 100% resistant to all tested antibiotics in 2014, whereas in 2015, resistance was found for AMP-NAL-TAX-SXT-FIX. The resistance phenotypes among S. flexneri isolates for the year 2014 and 2015 were AMP-SXT-NAL-NOR-FIX-TAX and AMP-NAL-SXT-TAX-NOR-FIX respectively. Screening for antimicrobial resistance genes in S. flexneri found dhfr1A, sulII, blaOXA, blaTEM, blaCTX-M-1,qnrB, qnrS and AmpC genes while S. sonnei were found to have only dhfr1A, sulII, blaCTX-M-1 and qnrS genes respectively. Antimicrobial resistance genes were predominantly seen in AMP-SXT-NAL and AMP-SXT-NAL-NOR resistance phenotypes. CONCLUSION: Shigella prevalence of 4.8% to 4.6% was documented between the years 2014 to 2015 in this study. We show evidence that resistance to commonly used antibiotics continues to increase among Shigella spp. in South India. The presence of qnrS and blaCTX-M-15 in the study isolates further indicates the threat of spreading resistance to quinolones and third-generation cephalosporins.
Authors: D P Muthuirulandi Sethuvel; N K Devanga Ragupathi; S Anandan; B Veeraraghavan Journal: Lett Appl Microbiol Date: 2016-11-28 Impact factor: 2.858
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