Literature DB >> 2893252

Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA.

D Cross1, M L Allende, R Y Krauss, M E Fuentes, G Kaltwasser, J Alvarez, N C Inestrosa.   

Abstract

In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.

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Year:  1987        PMID: 2893252     DOI: 10.1007/bf00444678

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN: 0301-4851            Impact factor:   2.316


  21 in total

Review 1.  The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.

Authors:  H Soreq
Journal:  CRC Crit Rev Biochem       Date:  1985

2.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors:  R D Palmiter
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

3.  Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.

Authors:  J B Gurdon; C D Lane; H R Woodland; G Marbaix
Journal:  Nature       Date:  1971-09-17       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Study of the 10-nm-filament fraction isolated during the standard microtubule preparation.

Authors:  A Delacourte; G Filliatreau; F Boutteau; G Biserte; J Schrevel
Journal:  Biochem J       Date:  1980-11-01       Impact factor: 3.857

6.  The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve.

Authors:  J S Pachter; R K Liem
Journal:  Dev Biol       Date:  1984-05       Impact factor: 3.582

7.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

8.  Neurofilament phosphorylation in development. A sign of axonal maturation?

Authors:  D Dahl; A Bignami
Journal:  Exp Cell Res       Date:  1986-01       Impact factor: 3.905

9.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162

10.  Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.

Authors:  N Geisler; E Kaufmann; S Fischer; U Plessmann; K Weber
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

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  1 in total

1.  Characterization of a tetrameric G4 form of acetylcholinesterase from bovine brain: a comparison with the dimeric G2 form of the electric organ.

Authors:  M E Fuentes; N C Inestrosa
Journal:  Mol Cell Biochem       Date:  1988-05       Impact factor: 3.396

  1 in total

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