Rogério Nogueira Soares1, Raylene A Reimer1,2, Zaid Alenezi1, Patricia K Doyle-Baker1,3, Juan Manuel Murias1. 1. 1 Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada. 2. 2 Department of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. 3. 3 Faculty of Environmental Design, University of Calgary, Calgary, AB, Canada.
Abstract
OBJECTIVE: To examine whether the near-infrared spectroscopy combined with vascular occlusion test technique could detect differences in vascular responsiveness during hyperglycaemia between normal-weight individuals and individuals with obesity. METHODS: A total of 16 normal-weight individuals (body mass index, 21.3 ± 1.7 kg/m2) and 13 individuals with obesity (body mass index, 34.4 ± 2.0 kg/m2) were submitted to five vascular occlusion tests (Pre, 30, 60, 90 and 120 min after glucose challenge). Vascular responsiveness was determined by the Slope 2 (Slope 2 StO2) and the area under the curve (StO2AUC) of oxygen saturation derived from near-infrared spectroscopy-vascular occlusion test. RESULTS: The Slope 2 StO2 increased from 1.07 ± 0.16%/s (Pre) to 1.53 ± 0.21%/s at 90 min ( p < 0.05) in the control group, while in obese it increased from 0.71 ± 0.09%/s (Pre) to 0.92 ± 0.14%/s at 60 min ( p < 0.05), and to 0.97 ± 0.10%/s ( p < 0.01) at 120 min after glucose ingestion. The StO2AUC decreased from 1729 ± 214% . sec (Pre) to 1259 ± 232% . sec at 60 min ( p < 0.05) and to 1034 ± 172% . sec at 90 min ( p < 0.05) in the normal-weight group, whereas it decreased at 90 min (637 ± 98% . sec; p < 0.05) and at 120 min (590 ± 93% . sec; p < 0.01) compared to 30 min (1232 ± 197% . sec) after glucose ingestion in individuals with obesity. CONCLUSION: Near-infrared spectroscopy-vascular occlusion test technique was capable of detecting differences in vascular responsiveness during hyperglycaemia between normal-weight individuals and individuals with obesity.
OBJECTIVE: To examine whether the near-infrared spectroscopy combined with vascular occlusion test technique could detect differences in vascular responsiveness during hyperglycaemia between normal-weight individuals and individuals with obesity. METHODS: A total of 16 normal-weight individuals (body mass index, 21.3 ± 1.7 kg/m2) and 13 individuals with obesity (body mass index, 34.4 ± 2.0 kg/m2) were submitted to five vascular occlusion tests (Pre, 30, 60, 90 and 120 min after glucose challenge). Vascular responsiveness was determined by the Slope 2 (Slope 2 StO2) and the area under the curve (StO2AUC) of oxygen saturation derived from near-infrared spectroscopy-vascular occlusion test. RESULTS: The Slope 2 StO2 increased from 1.07 ± 0.16%/s (Pre) to 1.53 ± 0.21%/s at 90 min ( p < 0.05) in the control group, while in obese it increased from 0.71 ± 0.09%/s (Pre) to 0.92 ± 0.14%/s at 60 min ( p < 0.05), and to 0.97 ± 0.10%/s ( p < 0.01) at 120 min after glucose ingestion. The StO2AUC decreased from 1729 ± 214% . sec (Pre) to 1259 ± 232% . sec at 60 min ( p < 0.05) and to 1034 ± 172% . sec at 90 min ( p < 0.05) in the normal-weight group, whereas it decreased at 90 min (637 ± 98% . sec; p < 0.05) and at 120 min (590 ± 93% . sec; p < 0.01) compared to 30 min (1232 ± 197% . sec) after glucose ingestion in individuals with obesity. CONCLUSION: Near-infrared spectroscopy-vascular occlusion test technique was capable of detecting differences in vascular responsiveness during hyperglycaemia between normal-weight individuals and individuals with obesity.
Authors: Rogerio N Soares; Juan M Murias; Flavia Saccone; Leopoldo Puga; Gustavo Moreno; Miguel Resnik; Gabriela F De Roia Journal: Physiol Rep Date: 2019-06
Authors: Felipe Mattioni Maturana; Philipp Schellhorn; Gunnar Erz; Christof Burgstahler; Manuel Widmann; Barbara Munz; Rogerio N Soares; Juan M Murias; Ansgar Thiel; Andreas M Nieß Journal: Eur J Appl Physiol Date: 2021-04-03 Impact factor: 3.078