D Morrone1, F Felice2, C Scatena3, A De Martino4, M L E Picoi2, N Mancini2, S Blasi4, M Menicagli5, R Di Stefano2, U Bortolotti4, A G Naccarato3, A Balbarini2. 1. Surgery, medicine, molecular and critical area Department - Cardiovascular disease Section 2, Pisa, Italy. Electronic address: doralisamorrone@gmail.com. 2. Surgery, medicine, molecular and critical area Department - Cardiovascular disease Section 2, Pisa, Italy. 3. Translational Re New Technologies in Medicine and Surgery Department, University of Pisa, Italy. 4. Surgery, medicine, molecular and critical area Department Cardiac-Surgery Section, University of Pisa, Italy. 5. Pisa Science Foundation, Pisa, Italy.
Abstract
BACKGROUND: Mobilization of endothelial progenitor cells (EPCs) into circulation from bone marrow in patients with acute myocardial infarction has strong scientific evidence; less is known about EPC mobilization in patients with stable coronary artery disease (CAD). The aim of this study was to investigate the association of stable ischemic heart disease with EPC levels in tissue and blood. METHODS: Fifty-five consecutive patients admitted to a single treatment center for valve or coronary artery bypass grafting (CABG) surgeries were included in the study. Blood samples were collected in the morning before surgery and analyzed by flow-cytometry to determine peripheral EPC levels (EPC/ml). Tissue EPC (CD34+VEGFR2+) levels were assessed on a right atrial appendage segment. RESULTS: Mean age was 76±5years, 48% were men, and 53% had CAD The number of CD34+ VEGFR2+ cells in the tissue of patients with CAD was significantly higher (p<0.005) and circulating EPC showed a tendency to be reduced by approximately 20% in peripheral blood of patients with CAD when compared to those without CAD. CONCLUSION: Patients with stable CAD had higher EPC density values (EPC/mm2) and were more likely to have lower EPC blood levels when compare with normal controls.
BACKGROUND: Mobilization of endothelial progenitor cells (EPCs) into circulation from bone marrow in patients with acute myocardial infarction has strong scientific evidence; less is known about EPC mobilization in patients with stable coronary artery disease (CAD). The aim of this study was to investigate the association of stable ischemic heart disease with EPC levels in tissue and blood. METHODS: Fifty-five consecutive patients admitted to a single treatment center for valve or coronary artery bypass grafting (CABG) surgeries were included in the study. Blood samples were collected in the morning before surgery and analyzed by flow-cytometry to determine peripheral EPC levels (EPC/ml). Tissue EPC (CD34+VEGFR2+) levels were assessed on a right atrial appendage segment. RESULTS: Mean age was 76±5years, 48% were men, and 53% had CAD The number of CD34+ VEGFR2+ cells in the tissue of patients with CAD was significantly higher (p<0.005) and circulating EPC showed a tendency to be reduced by approximately 20% in peripheral blood of patients with CAD when compared to those without CAD. CONCLUSION:Patients with stable CAD had higher EPC density values (EPC/mm2) and were more likely to have lower EPC blood levels when compare with normal controls.
Authors: Teng Su; Ke Huang; Kyle G Mathews; Valery F Scharf; Shiqi Hu; Zhenhua Li; Brianna N Frame; Jhon Cores; Phuong-Uyen Dinh; Michael A Daniele; Frances S Ligler; Ke Cheng Journal: ACS Biomater Sci Eng Date: 2020-10-05
Authors: Nicolas Herrera-Zelada; Ursula Zuñiga-Cuevas; Andres Ramirez-Reyes; Sergio Lavandero; Jaime A Riquelme Journal: Front Pharmacol Date: 2021-02-02 Impact factor: 5.810
Authors: Doralisa Morrone; Maria Elena Lucia Picoi; Francesca Felice; Andrea De Martino; Cristian Scatena; Paolo Spontoni; Antonio Giuseppe Naccarato; Rossella Di Stefano; Uberto Bortolotti; Massimo Dal Monte; Stefano Pini; Marianna Abelli; Alberto Balbarini Journal: Int J Mol Sci Date: 2021-11-28 Impact factor: 5.923