Literature DB >> 28904423

Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes.

Mohammad Abu Hena Mostofa Jamal1,2, Satya Priya Sharma1, Hea-Jong Chung1,3, Hyeon-Jin Kim4, Seong-Tshool Hong1, Seungkoo Lee5.   

Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

Entities:  

Keywords:  Colony PCR; DNA extraction; High throughput screening; Sequencing; Sonication

Year:  2017        PMID： 28904423      PMCID： PMC5574782          DOI： 10.1007/s12088-017-0665-1

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  18 in total

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6.  A rapid DNA isolation procedure for the identification of Campylobacter jejuni by the polymerase chain reaction.

Authors:  M D Englen; L C Kelley
Journal:  Lett Appl Microbiol       Date:  2000-12       Impact factor: 2.858

7.  Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study.

Authors:  P Azhahianambi; S Ghosh; C Ashok Kumar; V V S Suryanarayana
Journal:  Indian J Exp Biol       Date:  2008-10       Impact factor: 0.818

8.  Optimal cloning of PCR fragments by homologous recombination in Escherichia coli.

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Journal:  PLoS One       Date:  2015-03-16       Impact factor: 3.240

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Journal:  PLoS One       Date:  2013-05-29       Impact factor: 3.240

10.  Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens.

Authors:  Linxiang Chen; Ying Cai; Guangbiao Zhou; Xiaojun Shi; Jianhui Su; Guanwu Chen; Kun Lin
Journal:  PLoS One       Date:  2014-02-14       Impact factor: 3.240
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2.  Engineering Bacillus subtilis for the formation of a durable living biocomposite material.

Authors:  Sun-Young Kang; Anaya Pokhrel; Sara Bratsch; Joey J Benson; Seung-Oh Seo; Maureen B Quin; Alptekin Aksan; Claudia Schmidt-Dannert
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