Anna Maria Heikkinen1, Elmira Pakbaznejad Esmaeili1, Leena Kovanen1, Hellevi Ruokonen1, Kaisa Kettunen2, Jari Haukka3, Taina Tervahartiala4, Timo Sorsa5. 1. Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 2. FIMM, Institute for Molecular Medicine Finland, Helsinki, Uusimaa, Finland. 3. Docent, Department of Public Health, Clinicum, University of Helsinki, Uusimaa, Finland. 4. Docent, Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 5. Professor, Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland.
Abstract
INTRODUCTION: Periodontitis is a multifactorial infectious disease of the supporting tissues of teeth in which bacterial, genetic and lifestyle factors such as smoking have an important role. AIM: The aim was to examine if Bleeding On Probing (BOP ≥ 20%) and ≥ 4 mm deep pockets correlated with any suspicion of initial radiological findings of periodontitis and bone loss. We also investigated whether any pro-inflammatory-related candidate Single Nucleotide Polymorphisms (SNPs) were associated with any suspicion of radiological findings. MATERIALS AND METHODS: Altogether 47 generally healthy adolescent patients of one birth cohort had given their approval for their saliva samples to be used for DNA analysis. One participant was excluded after discrepant gender check. An oral radiologist analysed right and left bitewing radiographs of 47 patients. Clinical parameters such as BOP ≥ 20%, ≥ 4 mm pockets, Visible Plaque Index of all teeth (VPI%), as well as smoking habits were recorded. DNA was extracted and 71 SNPs from candidate genes for initial periodontitis were genotyped. The association between ≥ 4 mm pockets and BOP ≥ 20% with radiological findings and selected SNPs was modelled using logistic regression. RESULTS: Variants in Toll-Like Receptors 4 (TLR4) gene (rs498670) (OR=5.8, {CI95% 1.6-20.7}, p=0.02, FDR q-value=0.13) and TNFSF11 gene (rs2277438, OR=0.3 {CI95% 0.1-0.9}, p=0.002, FDR q-value=0.56) were associated with any suspicious radiological findings; however the significance vanished after False Discovery Rate analysis (FDR). The association between BOP ≥ 20% and any radiographic signs of periodontitis was found to be statistically significant, OR=1.6, CI 95% 1.0-2.4, p=0.04. CONCLUSION: Only TLR4 (rs498670) and TNFSF11 (rs2277438) genes were found to have a positive correlation with radiological findings suggestive of initial periodontitis after adjustment for smoking and visible plaque.
INTRODUCTION:Periodontitis is a multifactorial infectious disease of the supporting tissues of teeth in which bacterial, genetic and lifestyle factors such as smoking have an important role. AIM: The aim was to examine if Bleeding On Probing (BOP ≥ 20%) and ≥ 4 mm deep pockets correlated with any suspicion of initial radiological findings of periodontitis and bone loss. We also investigated whether any pro-inflammatory-related candidate Single Nucleotide Polymorphisms (SNPs) were associated with any suspicion of radiological findings. MATERIALS AND METHODS: Altogether 47 generally healthy adolescent patients of one birth cohort had given their approval for their saliva samples to be used for DNA analysis. One participant was excluded after discrepant gender check. An oral radiologist analysed right and left bitewing radiographs of 47 patients. Clinical parameters such as BOP ≥ 20%, ≥ 4 mm pockets, Visible Plaque Index of all teeth (VPI%), as well as smoking habits were recorded. DNA was extracted and 71 SNPs from candidate genes for initial periodontitis were genotyped. The association between ≥ 4 mm pockets and BOP ≥ 20% with radiological findings and selected SNPs was modelled using logistic regression. RESULTS: Variants in Toll-Like Receptors 4 (TLR4) gene (rs498670) (OR=5.8, {CI95% 1.6-20.7}, p=0.02, FDR q-value=0.13) and TNFSF11 gene (rs2277438, OR=0.3 {CI95% 0.1-0.9}, p=0.002, FDR q-value=0.56) were associated with any suspicious radiological findings; however the significance vanished after False Discovery Rate analysis (FDR). The association between BOP ≥ 20% and any radiographic signs of periodontitis was found to be statistically significant, OR=1.6, CI 95% 1.0-2.4, p=0.04. CONCLUSION: Only TLR4 (rs498670) and TNFSF11 (rs2277438) genes were found to have a positive correlation with radiological findings suggestive of initial periodontitis after adjustment for smoking and visible plaque.
Authors: Anna Maria Heikkinen; Riitta Pajukanta; Janne Pitkäniemi; Ulla Broms; Timo Sorsa; Markku Koskenvuo; Jukka H Meurman Journal: J Periodontol Date: 2008-11 Impact factor: 6.993
Authors: Anna M Heikkinen; Teija Raivisto; Ismo Räisänen; Taina Tervahartiala; Nagihan Bostanci; Timo Sorsa Journal: Clin Exp Dent Res Date: 2022-06-08
Authors: Jussi M Leppilahti; Ulla Harjunmaa; Jorma Järnstedt; Charles Mangani; Marcela Hernández; Taina Tervahartiala; Rodrigo Lopez; Ulla Ashorn; Per Ashorn; Dirk-Rolf Gieselmann; Timo Sorsa Journal: Diagnostics (Basel) Date: 2018-09-15