C J Tomás-Catalá1,2, M Collado-González1, D García-Bernal1, R E Oñate-Sánchez2, L Forner3, C Llena3, A Lozano3, P Castelo-Baz4, J M Moraleda1, F J Rodríguez-Lozano1,2. 1. Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain. 2. Faculty of Medicine, School of Dentistry, University of Murcia, Murcia, Spain. 3. Department of Stomatology, University de Valencia, Valencia, Spain. 4. University of Santiago de Compostela, Santiago de Compostela, Spain.
Abstract
AIM: To evaluate the biological effects in vitro of MTA-Angelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). METHODOLOGY: Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analysed by immunocytofluorescence and scanning electron microscopy, respectively. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and eluates were analysed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with the analysis of variance and Bonferroni or Tukey post-test (α < 0.05). RESULTS: Undiluted MTA-Ang, MTA-HP and NeoMTA-P displayed a significant increase in cell viability greater than that obtained using complete medium alone (control) (*P < 0.05; **P < 0.01; ***P < 0.001). Moreover, a cell migration assay revealed cell migration rates after incubation with extracts of MTA-Ang, MTA-HP and NeoMTA-P that were similar to levels obtained in the control group. In addition, stretched cytoskeletal F-actin fibres were detected in the cells treated with the three material extracts. SEM studies revealed a high degree of cell proliferation and attachment on all three materials. EDX analysis demonstrated similar weight percentages of C, O and Ca in all three materials, whilst other elements such as Al, Si and S were also found. CONCLUSIONS: MTA-Ang, MTA-HP and NeoMTA-P were associated with biological effects on hDPSCs in terms of cell proliferation, morphology, migration and attachment.
AIM: To evaluate the biological effects in vitro of MTA-Angelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). METHODOLOGY: Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analysed by immunocytofluorescence and scanning electron microscopy, respectively. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and eluates were analysed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with the analysis of variance and Bonferroni or Tukey post-test (α < 0.05). RESULTS: Undiluted MTA-Ang, MTA-HP and NeoMTA-P displayed a significant increase in cell viability greater than that obtained using complete medium alone (control) (*P < 0.05; **P < 0.01; ***P < 0.001). Moreover, a cell migration assay revealed cell migration rates after incubation with extracts of MTA-Ang, MTA-HP and NeoMTA-P that were similar to levels obtained in the control group. In addition, stretched cytoskeletal F-actin fibres were detected in the cells treated with the three material extracts. SEM studies revealed a high degree of cell proliferation and attachment on all three materials. EDX analysis demonstrated similar weight percentages of C, O and Ca in all three materials, whilst other elements such as Al, Si and S were also found. CONCLUSIONS: MTA-Ang, MTA-HP and NeoMTA-P were associated with biological effects on hDPSCs in terms of cell proliferation, morphology, migration and attachment.
Authors: Mar Collado-González; Sergio López-García; David García-Bernal; Ricardo E Oñate-Sánchez; Christopher J Tomás-Catalá; Jose M Moraleda; Adrián Lozano; Leopoldo Forner; Francisco J Rodríguez-Lozano Journal: Clin Oral Investig Date: 2019-01-25 Impact factor: 3.573
Authors: J L Sanz; S López-García; A Lozano; M P Pecci-Lloret; C Llena; J Guerrero-Gironés; F J Rodríguez-Lozano; L Forner Journal: Clin Oral Investig Date: 2020-07-10 Impact factor: 3.573
Authors: Arthur Dias Galarça; Wellington Luiz de Oliveira Da Rosa; Tiago Machado Da Silva; Giana da Silveira Lima; Neftalí Lenin Villarreal Carreño; Thiago Machado Pereira; Orlando Aguirre Guedes; Alvaro Henrique Borges; Adriana Fernandes da Silva; Evandro Piva Journal: Biomed Res Int Date: 2018-11-27 Impact factor: 3.411
Authors: C Llena; M Collado-González; D García-Bernal; R E Oñate-Sánchez; C M Martínez; J M Moraleda; F J Rodríguez-Lozano; L Forner Journal: Sci Rep Date: 2019-05-23 Impact factor: 4.379
Authors: Cláudio M A Ferreira; Luciana M Sassone; Alexia S Gonçalves; Jorge José de Carvalho; Christopher J Tomás-Catalá; David García-Bernal; Ricardo E Oñate-Sánchez; Francisco J Rodríguez-Lozano; Emmanuel João Nogueira Leal Silva Journal: Sci Rep Date: 2019-03-08 Impact factor: 4.379
Authors: James Ghilotti; José Luis Sanz; Sergio López-García; Julia Guerrero-Gironés; María P Pecci-Lloret; Adrián Lozano; Carmen Llena; Francisco Javier Rodríguez-Lozano; Leopoldo Forner; Gianrico Spagnuolo Journal: Materials (Basel) Date: 2020-05-10 Impact factor: 3.623