Xinyu Xu1, Lingling Bian1,2, Min Shen1, Xin Li1, Jing Zhu1, Shuang Chen1, Lei Xiao1, Qingqing Zhang1, Heng Chen1, Kuanfeng Xu1, Tao Yang3. 1. Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, People's Republic of China. 2. Department of Endocrinology, Yancheng City No.1 People's Hospital, Yancheng, Jiangsu Province, People's Republic of China. 3. Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, People's Republic of China. yangt@njmu.edu.cn.
Abstract
AIMS/HYPOTHESIS: Induction of antigen-specific immunological tolerance may provide an attractive immunotherapy in the NOD mouse model but the conditions that lead to the successful translation to human type 1 diabetes are limited. In this study, we covalently linked 500 nm carboxylated polystyrene beads (PSB) with a mixture of immunodominant HLA-A*02:01-restricted epitopes (peptides-PSB) that may have high clinical relevance in humans as they promote immune tolerance; we then investigated the effect of the nanoparticle-peptide complexes on T cell tolerance. METHODS: PSB-coupled mixtures of HLA-A*02:01-restricted epitopes were administered to HHD II mice via intravenous injection. The effects on delaying the course of the disease were verified in NOD.β2m null HHD mice. The diabetogenic HLA-A*02:01-restricted cytotoxic lymphocyte (CTL) responses to treatment with peptides-PSB were validated in individuals with type 1 diabetes. RESULTS: We showed that peptides-PSB could induce antigen-specific tolerance in HHD II mice. The protective immunological mechanisms were mediated through the function of CD4+CD25+ regulatory T cells, suppressive T cell activation and T cell anergy. Furthermore, the peptides-PSB induced an activation and accumulation of regulatory T cells and CD11c+ dendritic cells through a rapid production of CD169+ macrophage-derived C-C motif chemokine 22 (CCL22). Peptides-PSB also prevented diabetes in 'humanised' NOD.β2m null HHD mice and suppressed pathogenic CTL responses in people with type 1 diabetes. CONCLUSIONS/ INTERPRETATION: Our findings demonstrate for the first time the potential for using multipeptide-PSB complexes to induce T cell tolerance and halt the autoimmune process. These findings represent a promising platform for an antigen-specific tolerance strategy in type 1 diabetes and highlight a mechanism through which metallophilic macrophages mediate the early cell-cell interactions required for peptides-PSB-induced immune tolerance.
AIMS/HYPOTHESIS: Induction of antigen-specific immunological tolerance may provide an attractive immunotherapy in the NOD mouse model but the conditions that lead to the successful translation to humantype 1 diabetes are limited. In this study, we covalently linked 500 nm carboxylated polystyrene beads (PSB) with a mixture of immunodominant HLA-A*02:01-restricted epitopes (peptides-PSB) that may have high clinical relevance in humans as they promote immune tolerance; we then investigated the effect of the nanoparticle-peptide complexes on T cell tolerance. METHODS: PSB-coupled mixtures of HLA-A*02:01-restricted epitopes were administered to HHD II mice via intravenous injection. The effects on delaying the course of the disease were verified in NOD.β2m null HHDmice. The diabetogenic HLA-A*02:01-restricted cytotoxic lymphocyte (CTL) responses to treatment with peptides-PSB were validated in individuals with type 1 diabetes. RESULTS: We showed that peptides-PSB could induce antigen-specific tolerance in HHD II mice. The protective immunological mechanisms were mediated through the function of CD4+CD25+ regulatory T cells, suppressive T cell activation and T cell anergy. Furthermore, the peptides-PSB induced an activation and accumulation of regulatory T cells and CD11c+ dendritic cells through a rapid production of CD169+ macrophage-derived C-C motif chemokine 22 (CCL22). Peptides-PSB also prevented diabetes in 'humanised' NOD.β2m null HHDmice and suppressed pathogenic CTL responses in people with type 1 diabetes. CONCLUSIONS/ INTERPRETATION: Our findings demonstrate for the first time the potential for using multipeptide-PSB complexes to induce T cell tolerance and halt the autoimmune process. These findings represent a promising platform for an antigen-specific tolerance strategy in type 1 diabetes and highlight a mechanism through which metallophilic macrophages mediate the early cell-cell interactions required for peptides-PSB-induced immune tolerance.
Entities:
Keywords:
Antigen-specific tolerance; Humanised mice; Immunotherapy; Nanoparticles; Type 1 diabetes
Authors: Kelan A Hlavaty; Derrick P McCarthy; Eiji Saito; Woon Teck Yap; Stephen D Miller; Lonnie D Shea Journal: Biomaterials Date: 2015-10-21 Impact factor: 12.479
Authors: Bingye Han; Pau Serra; Abdelaziz Amrani; Jun Yamanouchi; Athanasius F M Marée; Leah Edelstein-Keshet; Pere Santamaria Journal: Nat Med Date: 2005-05-22 Impact factor: 53.440
Authors: Marijke Niens; Alexandra E Grier; Michele Marron; Thomas W H Kay; Dale L Greiner; David V Serreze Journal: Diabetes Date: 2011-02-23 Impact factor: 9.461
Authors: Elana Ben-Akiva; Savannah Est Witte; Randall A Meyer; Kelly R Rhodes; Jordan J Green Journal: Biomater Sci Date: 2018-12-18 Impact factor: 6.843