Literature DB >> 28880021

Long-term expression of melanopsin and channelrhodopsin causes no gross alterations in the dystrophic dog retina.

B Ameline1, K-T Tshilenge1, M Weber2, M Biget1, L Libeau1,2, R Caplette3,4,5, A Mendes-Madeira1, N Provost1, C Guihal1, S Picaud3,4,5, P Moullier1, V Pichard1, T Cronin1, C Isiegas1.   

Abstract

Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in a large animal model of retinal degeneration, the RPE65-deficient Briard dog model of Leber congenital amaurosis. Intravitreal injection of adeno-associated virus vectors expressing channelrhodopsin and melanopsin had no effect on retinal thickness over a 16-month period post injection. Our data support the safety of the optogenetic approach for the treatment of blindness.

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Year:  2017        PMID： 28880021     DOI： 10.1038/gt.2017.63

Source DB:  PubMed          Journal:  Gene Ther        ISSN： 0969-7128            Impact factor:   5.250


  31 in total

1.  Melanopsin-expressing ganglion cells in primate retina signal colour and irradiance and project to the LGN.

Authors:  Dennis M Dacey; Hsi-Wen Liao; Beth B Peterson; Farrel R Robinson; Vivianne C Smith; Joel Pokorny; King-Wai Yau; Paul D Gamlin
Journal:  Nature       Date:  2005-02-17       Impact factor: 49.962

2.  Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats.

Authors:  Hiroshi Tomita; Eriko Sugano; Hitomi Isago; Teru Hiroi; Zhuo Wang; Emi Ohta; Makoto Tamai
Journal:  Exp Eye Res       Date:  2009-12-27       Impact factor: 3.467

3.  The effect of photoreceptor degeneration on ganglion cell morphology.

Authors:  E E O'Brien; U Greferath; E L Fletcher
Journal:  J Comp Neurol       Date:  2014-04-01       Impact factor: 3.215

4.  Restoration of vision in the pde6β-deficient dog, a large animal model of rod-cone dystrophy.

Authors:  Lolita Petit; Elsa Lhériteau; Michel Weber; Guylène Le Meur; Jack-Yves Deschamps; Nathalie Provost; Alexandra Mendes-Madeira; Lyse Libeau; Caroline Guihal; Marie-Anne Colle; Philippe Moullier; Fabienne Rolling
Journal:  Mol Ther       Date:  2012-07-24       Impact factor: 11.454

5.  Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy.

Authors:  E Sugano; H Isago; Z Wang; N Murayama; M Tamai; H Tomita
Journal:  Gene Ther       Date:  2010-10-28       Impact factor: 5.250

6.  Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity.

Authors:  S Hattar; H W Liao; M Takao; D M Berson; K W Yau
Journal:  Science       Date:  2002-02-08       Impact factor: 47.728

7.  Changes in morphology of retinal ganglion cells with eccentricity in retinal degeneration.

Authors:  E E Anderson; U Greferath; E L Fletcher
Journal:  Cell Tissue Res       Date:  2015-12-16       Impact factor: 5.249

8.  Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.

Authors:  Georg Nagel; Tanjef Szellas; Wolfram Huhn; Suneel Kateriya; Nona Adeishvili; Peter Berthold; Doris Ollig; Peter Hegemann; Ernst Bamberg
Journal:  Proc Natl Acad Sci U S A       Date:  2003-11-13       Impact factor: 11.205

9.  Restoring the ON Switch in Blind Retinas: Opto-mGluR6, a Next-Generation, Cell-Tailored Optogenetic Tool.

Authors:  Michiel van Wyk; Justyna Pielecka-Fortuna; Siegrid Löwel; Sonja Kleinlogel
Journal:  PLoS Biol       Date:  2015-05-07       Impact factor: 8.029

10.  Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.

Authors:  Therese Cronin; Luk H Vandenberghe; Péter Hantz; Josephine Juttner; Andreas Reimann; Agota-Enikő Kacsó; Rachel M Huckfeldt; Volker Busskamp; Hubertus Kohler; Pamela S Lagali; Botond Roska; Jean Bennett
Journal:  EMBO Mol Med       Date:  2014-09       Impact factor: 12.137
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  3 in total

1.  A 2020 vision of ocular gene therapy.

Authors:  Robert E MacLaren
Journal:  Gene Ther       Date:  2020-06-29       Impact factor: 5.250

2.  Restoration of Vision and Retinal Responses After Adeno-Associated Virus-Mediated Optogenetic Therapy in Blind Dogs.

Authors:  Sergei Nikonov; Puya Aravand; Arkady Lyubarsky; Roman Nikonov; Angela J Luo; Zhangyong Wei; Albert M Maguire; Nicholas T Phelps; Ivan Shpylchak; Keirnan Willett; Tomas S Aleman; Rachel M Huckfeldt; Pavitra S Ramachandran; Jean Bennett
Journal:  Transl Vis Sci Technol       Date:  2022-05-02       Impact factor: 3.048

Review 3.  Optogenetic Gene Therapy for the Degenerate Retina: Recent Advances.

Authors:  Michelle E McClements; Federica Staurenghi; Robert E MacLaren; Jasmina Cehajic-Kapetanovic
Journal:  Front Neurosci       Date:  2020-11-11       Impact factor: 4.677
  3 in total

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