| Literature DB >> 28873762 |
Fuhua Li1, Bin Zhang1, Gu Chen2, Xiong Fu3.
Abstract
To explore the comprehensive phenolic profile of mulberry (Morus alba L.) fruit and reveal the novel anti-diabetic constituents, mulberry polyphenols (MP) were fractionated through gradient ethanol elution and subjected to composition and bioactivity analysis. Forty-seven phenolic compounds were identified and quantified through UHPLC-HR-ESI-TOF-MS/MS, including twenty-one newly identified compounds such as delphinidin 3-rutinoside-5-glucoside and cyanidin 3-rutinoside dimer. The antioxidant activity and anti-diabetic potential of fractionated MP were analyzed in vitro and ex vivo with HepG2 and pancreatic β-cell RIN-m5F. Hierarchical cluster analysis and Pearson correlation coefficients revealed compounds such as syringic acid and galloylcyanidin-glycoside contributed most to inhibit α-glucosidase activity. Quercetin and cyanidin-glycosides were essential for cellular antioxidant activity. Dihydroquercetin and 1,5-dicaffeoylquinic acid were newly identified and validated as the main contributors after rutin to protect RIN-m5F cells against glucotoxicity. Such findings suggest the promising role of MP for type II diabetics and lay the foundation of further utilization and investigation.Entities:
Keywords: 1,5-Dicaffeoylquinic acid (PubChem CID: 122,685); Caffeic acid (PubChem CID: 689043); Correlation analysis; Cyanidin 3-glucoside (PubChem CID: 44256715); Cyanidin 3-rutinoside (PubChem CID: 441674); Cyanidin 3-sophoroside (PubChem CID: 44256720); Hierarchical cluster analysis; Kaempferol-3-rutinoside (PubChem CID: 5318767); Mulberry polyphenols; Pancreatic β-cell RIN-m5F; Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805); Sinapic acid (PubChem CID: 637775); UHPLC-HR-ESI-TOF-MS/MS
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Year: 2017 PMID： 28873762 DOI： 10.1016/j.foodres.2017.06.052
Source DB: PubMed Journal: Food Res Int ISSN： 0963-9969 Impact factor: 6.475