Literature DB >> 28868330

Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans.

Kelly H Oh1, Hongkyun Kim1.   

Abstract

Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay is based on the correlation of the rate of neurotransmitter release with the rate of paralysis. In this protocol, we describe a method for simultaneously assessing the aldicarb sensitivity of animals with different genotypes.

Entities:  

Keywords:  Acetylcholinesterase; Aldicarb; C. elegans; Synaptic transmission

Year:  2017        PMID： 28868330      PMCID： PMC5580937          DOI： 10.21769/BioProtoc.2400

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction.

Authors:  J E Richmond; E M Jorgensen
Journal:  Nat Neurosci       Date:  1999-09       Impact factor: 24.884

Review 2.  Electrophysiological recordings from the neuromuscular junction of C. elegans.

Authors:  Janet E Richmond
Journal:  WormBook       Date:  2006-10-06

Review 3.  Maintenance of C. elegans.

Authors:  Theresa Stiernagle
Journal:  WormBook       Date:  2006-02-11

Review 4.  C. elegans locomotion: small circuits, complex functions.

Authors:  Mei Zhen; Aravinthan D T Samuel
Journal:  Curr Opin Neurobiol       Date:  2015-04-04       Impact factor: 6.627

5.  A conserved postsynaptic transmembrane protein affecting neuromuscular signaling in Caenorhabditis elegans.

Authors:  Paula M Loria; Jonathan Hodgkin; Oliver Hobert
Journal:  J Neurosci       Date:  2004-03-03       Impact factor: 6.167

Review 6.  Neurotransmitter release: the last millisecond in the life of a synaptic vesicle.

Authors:  Thomas C Südhof
Journal:  Neuron       Date:  2013-10-30       Impact factor: 17.173

Review 7.  Acetylcholine.

Authors:  James B Rand
Journal:  WormBook       Date:  2007-01-30

8.  ERG-28 controls BK channel trafficking in the ER to regulate synaptic function and alcohol response in C. elegans.

Authors:  Kelly H Oh; James J Haney; Xiaohong Wang; Chiou-Fen Chuang; Janet E Richmond; Hongkyun Kim
Journal:  Elife       Date:  2017-02-07       Impact factor: 8.140
  8 in total
  8 in total

1.  Blockade and reversal of swimming-induced paralysis in C. elegans by the antipsychotic and D2-type dopamine receptor antagonist azaperone.

Authors:  Osama Refai; Randy D Blakely
Journal:  Neurochem Int       Date:  2018-05-22       Impact factor: 3.921

2.  Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors.

Authors:  Kyoko Chiba; Hironori Takahashi; Min Chen; Hiroyuki Obinata; Shogo Arai; Koichi Hashimoto; Toshiyuki Oda; Richard J McKenney; Shinsuke Niwa
Journal:  Proc Natl Acad Sci U S A       Date:  2019-08-27       Impact factor: 11.205

3.  Assessing motor-related phenotypes of Caenorhabditis elegans with the wide field-of-view nematode tracking platform.

Authors:  Mandy Koopman; Quentin Peter; Renée I Seinstra; Michele Perni; Michele Vendruscolo; Christopher M Dobson; Tuomas P J Knowles; Ellen A A Nollen
Journal:  Nat Protoc       Date:  2020-05-20       Impact factor: 13.491

4.  Methods to Quantify and Relate Axonal Transport Defects to Changes in C. elegans Behavior.

Authors:  Syed Nooruzuha Barmaver; Muniesh Muthaiyan Shanmugam; Oliver Ingvar Wagner
Journal:  Methods Mol Biol       Date:  2022

5.  The C-terminal of CASY-1/Calsyntenin regulates GABAergic synaptic transmission at the Caenorhabditis elegans neuromuscular junction.

Authors:  Shruti Thapliyal; Amruta Vasudevan; Yongming Dong; Jihong Bai; Sandhya P Koushika; Kavita Babu
Journal:  PLoS Genet       Date:  2018-03-12       Impact factor: 5.917

6.  Cholinergic signaling at the body wall neuromuscular junction distally inhibits feeding behavior in Caenorhabditis elegans.

Authors:  Patricia G Izquierdo; Fernando Calahorro; Thibana Thisainathan; James H Atkins; Johanna Haszczyn; Christian J Lewis; John E H Tattersall; A Christopher Green; Lindy Holden-Dye; Vincent O'Connor
Journal:  J Biol Chem       Date:  2021-12-03       Impact factor: 5.157

7.  Neuroligin-mediated neurodevelopmental defects are induced by mitochondrial dysfunction and prevented by lutein in C. elegans.

Authors:  Silvia Maglioni; Alfonso Schiavi; Marlen Melcher; Vanessa Brinkmann; Zhongrui Luo; Anna Laromaine; Nuno Raimundo; Joel N Meyer; Felix Distelmaier; Natascia Ventura
Journal:  Nat Commun       Date:  2022-05-12       Impact factor: 17.694

8.  Pentylenetetrazole (PTZ)-induced Convulsion Assay to Determine GABAergic Defects in Caenorhabditis elegans.

Authors:  Shruti Thapliyal; Kavita Babu
Journal:  Bio Protoc       Date:  2018-09-05
  8 in total

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