Maria Teresa Segura1, Hans Demmelmair2, Susanne Krauss-Etschmann3, Petra Nathan3, Stefan Dehmel3, Maria Carmen Padilla4, Ricardo Rueda5, Berthold Koletzko6, Cristina Campoy7. 1. EURISTIKOS Excellence Centre for Paediatric Research, University of Granada, Spain; Ludwig-Maximilians-Universität München, Div. Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany; Department of Pediatrics, University of Granada, Spain. 2. Ludwig-Maximilians-Universität München, Div. Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany. 3. Comprehensive Pneumology Center (CPC), Ludwig-Maximilians-University, and Helmholtz Center Munich, Institute of Lung Biology and Disease (iLBD), Helmholtz Center Munich, Member of the German Center for Lung Research (DZL), Germany. 4. Obstetrics and Gynecology Service, Clinical University Hospital San Cecilio, Granada, Spain; Department of Obstetrics and Gynecology, University of Granada, Spain. 5. R&D Department, Abbott Laboratories, Granada, Spain. 6. Ludwig-Maximilians-Universität München, Div. Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, Munich, Germany. Electronic address: office.koletzko@med.lmu.de. 7. EURISTIKOS Excellence Centre for Paediatric Research, University of Granada, Spain; Department of Pediatrics, University of Granada, Spain.
Abstract
INTRODUCTION: Placental fatty acid (FA) uptake and metabolism depend on maternal supply which may be altered when women have a high pre-pregnancy body mass index (BMI) or develop gestational diabetes (GDM). Consequently, an impaired FA transport to the fetus may negatively affect fetal development. While placental adaptation of maternal-fetal glucose transfer in mild GDM has been described, knowledge on placental FA acid metabolism and possible adaptations in response to maternal obesity or GDM is lacking. We aimed to analyze the FA composition and the expression of key genes involved in FA uptake and metabolism in placentas from women with pre-pregnancy normal weight (18.5 ≤ BMI<25 kg/m2), overweight (25 ≤ BMI<30 kg/m2), obesity (BMI ≥ 30 kg/m2), and lean pregnant women with GDM. METHODS: Placental FA content was determined by gas liquid chromatography. Placental mRNA expression of FA transport proteins (FATP1, FATP4, FATP6), FA binding proteins (FABP3, FABP4, FABP7), FA translocase (FAT/CD36) and enzymes (Endothelial lipase (EL) and lipoprotein lipase (LPL)) were quantified by qRT-PCR. RESULTS: High pre-pregnancy BMI and GDM were associated with decreased placental FATP1, FATP4, EL and increased FAT/CD36 and FATP6 expressions. LPL mRNA levels and placental total FA content were similar among groups. Specific FA, including some long-chain polyunsaturated FA, were altered. DISCUSSION: Our results demonstrate that high pre-pregnancy BMI or GDM independently alter mRNA expression levels of genes involved in FA uptake and metabolism and the placental FA profile, which could affect fetal development and long-term health.
INTRODUCTION:Placental fatty acid (FA) uptake and metabolism depend on maternal supply which may be altered when women have a high pre-pregnancy body mass index (BMI) or develop gestational diabetes (GDM). Consequently, an impaired FA transport to the fetus may negatively affect fetal development. While placental adaptation of maternal-fetal glucose transfer in mild GDM has been described, knowledge on placental FA acid metabolism and possible adaptations in response to maternal obesity or GDM is lacking. We aimed to analyze the FA composition and the expression of key genes involved in FA uptake and metabolism in placentas from women with pre-pregnancy normal weight (18.5 ≤ BMI<25 kg/m2), overweight (25 ≤ BMI<30 kg/m2), obesity (BMI ≥ 30 kg/m2), and lean pregnant women with GDM. METHODS: Placental FA content was determined by gas liquid chromatography. Placental mRNA expression of FA transport proteins (FATP1, FATP4, FATP6), FA binding proteins (FABP3, FABP4, FABP7), FA translocase (FAT/CD36) and enzymes (Endothelial lipase (EL) and lipoprotein lipase (LPL)) were quantified by qRT-PCR. RESULTS: High pre-pregnancy BMI and GDM were associated with decreased placental FATP1, FATP4, EL and increased FAT/CD36 and FATP6 expressions. LPL mRNA levels and placental total FA content were similar among groups. Specific FA, including some long-chain polyunsaturated FA, were altered. DISCUSSION: Our results demonstrate that high pre-pregnancy BMI or GDM independently alter mRNA expression levels of genes involved in FA uptake and metabolism and the placental FA profile, which could affect fetal development and long-term health.
Authors: Jenney Liu; Icksoo Lee; Han-Zhong Feng; Sujay S Galen; Philipp P Hüttemann; Guy A Perkins; J-P Jin; Maik Hüttemann; Moh H Malek Journal: J Strength Cond Res Date: 2018-05 Impact factor: 3.775
Authors: Samuel Furse; Sara L White; Claire L Meek; Benjamin Jenkins; Clive J Petry; Matias C Vieira; Susan E Ozanne; David B Dunger; Lucilla Poston; Albert Koulman Journal: Mol Omics Date: 2019-12-02