| Literature DB >> 28825728 |
Jun Xiao1, Run Jin1, Xiang Yu1, Max Shen1, John D Wagner1, Armaan Pai1, Claire Song1, Michael Zhuang1, Samantha Klasfeld1, Chongsheng He2, Alexandre M Santos3, Chris Helliwell4, Jose L Pruneda-Paz5, Steve A Kay6, Xiaowei Lin7, Sujuan Cui7, Meilin Fernandez Garcia8, Oliver Clarenz9, Justin Goodrich9, Xiaoyu Zhang3, Ryan S Austin10,11, Roberto Bonasio2, Doris Wagner1.
Abstract
Disruption of gene silencing by Polycomb protein complexes leads to homeotic transformations and altered developmental-phase identity in plants. Here we define short genomic fragments, known as Polycomb response elements (PREs), that direct Polycomb repressive complex 2 (PRC2) placement at developmental genes regulated by silencing in Arabidopsis thaliana. We identify transcription factor families that bind to these PREs, colocalize with PRC2 on chromatin, physically interact with and recruit PRC2, and are required for PRC2-mediated gene silencing in vivo. Two of the cis sequence motifs enriched in the PREs are cognate binding sites for the identified transcription factors and are necessary and sufficient for PRE activity. Thus PRC2 recruitment in Arabidopsis relies in large part on binding of trans-acting factors to cis-localized DNA sequence motifs.Entities:
Mesh：
Substances：
Year: 2017 PMID： 28825728 DOI： 10.1038/ng.3937
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330