Literature DB >> 28825546

A Validated Capillary Electrophoretic Method for the Determination of Olopatadine and Its Application to a Pharmaceutical Preparation of Eye Drops.

Tufan Güray1, Tugba Turan1, Muzaffer Tunçel2, Ulku Dilek Uysal3.   

Abstract

A validated rapid and sensitive capillary zone electrophoretic method for the determination of olopatadine hydrochloride (OLO) is described. Optimum conditions were found: 20 mmol/L sodium tetraborate buffer, acetonitrile 15% (v/v), 10 mmol/L NaCl at pH 9.5, with 25 kV of applied potential, injection time of 10 s at 5 × 103 N/m2, at a wavelength of 205 nm, and fixed temperature of 30°C. The calibration curve was linear in the range of 1.13 × 10-5 mol/L (4.22 μg/mL) to 5.65 × 10-5 mol/L (21.12 μg/mL), with R = 0.9995 for interday precision. LOD and LOQ values were 1.58 × 10-6 (0.58 μg/mL) and 4.78 × 10-6 mol/L (1.75 μg/mL), respectively. Precision values were 1.10-1.97% for intraday and 1.41% for interday RSDs. Accuracy was tested by preparing a synthetic mixture whose composition was similar to the pharmaceutical preparation for Patanol. The RSDs of the recovery values (98.2%) were between 0.42 and 0.65% and the amount of OLO found was 1.09 mg/mL. The result was within the requirements of USP 31-NF 26. Therefore, this validated method is suggested for routine analysis for the determination of OLO in laboratories.

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Year:  2017        PMID: 28825546     DOI: 10.5740/jaoacint.16-0201

Source DB:  PubMed          Journal:  J AOAC Int        ISSN: 1060-3271            Impact factor:   1.913


  3 in total

1.  Intrinsic Stability Study and Forced Degradation Profiling of Olopatadine Hydrochloride by RP-HPLC-DAD-HRMS Method.

Authors:  Pawan Kumar Basniwal; Deepti Jain
Journal:  Turk J Pharm Sci       Date:  2019-11-11

2.  Simultaneous determination of four phenolic acids in traditional Chinese medicine by capillary electrophoresis-chemiluminescence.

Authors:  Shuopeng Yang; Yanzhen Han; Kairui Wang; Yu Wang; Liping Li; Nan Li; Xiangdong Xu
Journal:  RSC Adv       Date:  2021-10-20       Impact factor: 4.036

3.  A validated capillary electrophoretic method for the determination of indacaterol and its application to a pharmaceutical preparation.

Authors:  Tufan Güray; Muzaffer Tunçel; Ulku Dilek Uysal
Journal:  J Food Drug Anal       Date:  2017-09-22       Impact factor: 6.157

  3 in total

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