Literature DB >> 28824239

Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol.

Rahul Naithani1, Nitin Dayal2, Gaurav Dixit1.   

Abstract

Hematopoietic stem cell transplantation is curative therapy in benign and malignant diseases. Adequate stem cell dose is one of the most important marker of engraftment. Several methods have been developed to enumerate CD34+ cells. We present our data on 147 samples analysis. There was a clear linear correlation between two methods. Both methods were effective. Both single vs dual platform analysis yield similar results. Single platform analysis is easier to perform. In terms of cost reduction dual platform analysis is better.

Entities:  

Keywords:  Bone marrow transplantation; Enumeration; Flowcytometry; Hematopoietic stem cells; ISHAGE

Year:  2016        PMID: 28824239      PMCID: PMC5544640          DOI: 10.1007/s12288-016-0749-9

Source DB:  PubMed          Journal:  Indian J Hematol Blood Transfus        ISSN: 0971-4502            Impact factor:   0.900


  12 in total

1.  Single- versus dual-platform assays for human CD34+ cell enumeration.

Authors:  I L Barbosa; M E Sousa; M I Godinho; F Sousa; A Carvalhais
Journal:  Cytometry       Date:  1999-12-15

2.  Comparison of three methods of CD34+ cell enumeration in peripheral blood: dual-platform ISHAGE protocol versus single-platform, versus microvolume fluorimetry.

Authors:  P Chapple; H M Prince; D Wall; R Filshie; D Haylock; M Quinn; M Bretell; D Venter
Journal:  Cytotherapy       Date:  2000       Impact factor: 5.414

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Authors:  G Robinson; L Morgan; M Evans; S McDermott; S Pereira; M Wansbrough-Jones; G Griffin
Journal:  Lancet       Date:  1992-08-22       Impact factor: 79.321

Review 4.  Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis.

Authors:  J W Gratama; A Orfao; D Barnett; B Brando; A Huber; G Janossy; H E Johnsen; M Keeney; G E Marti; F Preijers; G Rothe; S Serke; D R Sutherland; C E Van der Schoot; G Schmitz; S Papa
Journal:  Cytometry       Date:  1998-06-15

5.  CD34+ cell enumeration by flow cytometry: a comparison of systems and methodologies.

Authors:  Alain Ngoma; Shunnichi Saito; Hitoshi Ohto; Kazuhiko Ikeda; Hiroyasu Yasuda; Kinuyo Kawabata; Takahiro Kanno; Atsushi Kikuta; Kazuhiro Mochizuki; Kenneth E Nollet
Journal:  Arch Pathol Lab Med       Date:  2011-07       Impact factor: 5.534

6.  Quality assurance of stem cell enumeration by flow cytometry. Canadian QASE Study Group.

Authors:  I Chin-Yee; L Anderson; M Keeney; D R Sutherland
Journal:  Cytometry       Date:  1997-12-15

7.  Performance evaluation of the FACSCount System: a dedicated system for clinical cellular analysis.

Authors:  K Strauss; I Hannet; S Engels; A Shiba; D M Ward; S Ullery; M G Jinguji; J Valinsky; D Barnett; A Orfao; L Kestens
Journal:  Cytometry       Date:  1996-03-15

8.  Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering.

Authors:  M Keeney; I Chin-Yee; K Weir; J Popma; R Nayar; D R Sutherland
Journal:  Cytometry       Date:  1998-04-15

9.  Immunologic differentiation of absolute lymphocyte count with an integrated flow cytometric system: a new concept for absolute T cell subset determinations.

Authors:  T J Mercolino; M C Connelly; E J Meyer; M D Knight; J W Parker; G T Stelzer; G DeChirico
Journal:  Cytometry       Date:  1995-03-15

10.  The composition of CD34 subpopulations differs between bone marrow, blood and cord blood.

Authors:  G Fritsch; M Stimpfl; M Kurz; D Printz; P Buchinger; G Fischmeister; P Hoecker; H Gadner
Journal:  Bone Marrow Transplant       Date:  1996-02       Impact factor: 5.483

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  2 in total

1.  Autologous Hematopoietic Stem Cell Transplantation for Multiple Myeloma in India.

Authors:  Rahul Naithani; Nitin Dayal; Reeta Rai; Sangeeta Pathak; Manoj Singh
Journal:  Indian J Hematol Blood Transfus       Date:  2018-03-22       Impact factor: 0.900

2.  Innovator Filgrastim versus Generic Filgrastim in Hematopoietic Stem Cell Transplantation Mobilization.

Authors:  Sadik Husian; Preethi Jeyaraman; S K Gupta; Reeta Rai; Sangeeta Pathak; Nitin Dayal; Rahul Naithani
Journal:  South Asian J Cancer       Date:  2021-09-04
  2 in total

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