Intradialytic Central Venous Oxygen Saturation is Associated with Clinical Outcomes in Hemodialysis Patients.

Central venous oxygen saturation (ScvO2) in the superior vena cava is predominantly determined by cardiac output, arterial oxygen content, and oxygen consumption by the upper body. While abnormal ScvO2 levels are associated with morbidity and mortality in non-uremic populations, ScvO2 has received little attention in hemodialysis patients. From 1/2012 to 8/2015, 232 chronic hemodialysis patients with central venous catheters as vascular access had their ScvO2 monitored during a 6-month baseline period and followed for up to 36 months. Patients were stratified into upper and lower two tertiles by a ScvO2 of 61.1%. Survival analysis employed Kaplan-Meier curves and adjusted Cox proportional hazards models. Patients in the lower tertiles of ScvO2 were older, had longer hemodialysis vintage, lower systolic blood pressure, lower ultrafiltration rates, higher leukocyte counts and neutrophil-to-lymphocyte ratios. Kaplan-Meier analysis indicated a shorter survival time in the lower tertiles of ScvO2 (P = 0.005, log-rank test). In adjusted Cox analysis, a 1 percent point decrease in mean ScvO2 was associated with a 4% increase in mortality (HR 1.04 [95% CI 1.01-1.08], P = 0.044), indicating that low ScvO2 is associated with poor outcomes. Research on the relative contributions of cardiac output and other factors is warranted to further elucidate the pathophysiology underlying this novel finding.

Introduction

The mortality rate of hemodialysis (HD) patients is elevated compared to the normal population[1]. The primary cause of mortality is cardiovascular disease (CVD), and there is evidence that the mechanism for CVD in HD patients differ from the traditional CVD risk factors in the general population[2, 3]. High ultrafiltration rates (UFR), episodes of intradialytic hypotension, presence of congestive heart failure (CHF) and left ventricular hypertrophy (LVH) are some of the factors that have been associated with increased mortality[4, 5]. Additionally nocturnal hypoxemia in HD patients has been demonstrated to be associated with worse cardiovascular outcomes[6, 7].

Mixed venous oxygen saturation (SmvO2) and central venous oxygen saturation (ScvO2) have been used in critical care to guide fluid resuscitation[8]. SmvO2 is the oxygen saturation in the pulmonary artery, which receives blood from the superior vena cava, the inferior vena cava, and the coronary sinus, and therefore reflects – in the absence of arterial venous shunts – the aggregated effects of oxygen delivery to and utilization by the entire body. ScvO2 from upper body central venous catheters (CVC) is the oxygen saturation of blood in the superior vena cava, which reflects the aggregate of oxygen delivery to and utilization by the upper body. Although resting SmvO2 and ScvO2 differ due to the higher oxygen extraction in the upper body, the time trends of SmvO2 and ScvO2 are comparable under most circumstances[9-11]. While the measurement of SmvO2 requires pulmonary artery catheterization, ScvO2 can be more easily obtained from a CVC.

ScvO2 is determined by oxygen delivery to and oxygen consumption of the arms, head, and upper portion of the torso; the former depends on the arterial blood oxygen content and the cardiac output (CO). At rest with stable arterial oxygen saturation (SaO2), hemoglobin, and tissue oxygen consumption, ScvO2 can serve as a surrogate of CO. Poor oxygen delivery can be caused by decreased CO, e.g. from CHF or reduced cardiac preload, or decreased arterial oxygen content, e.g. due to anemia or hypoxemic states. Oxygen consumption is determined by metabolic status and is altered in sepsis, fever, exercise and sedation[12]. ScvO2 in the general population is poorly defined, as obtaining this measurement requires a CVC, and patients who require CVC placement are generally significantly ill. One study in healthy subjects found a ScvO2 of 76.8 ± 5.2% during cardiac catheterization[13].

Studies in non-uremic populations, have found that abnormal ScvO2 levels are associated with worse morbidity and mortality[8, 14–18]. ScvO2 levels in HD patients have not been well described. In patients who have ESRD with CVC as vascular access, ScvO2 can be easily and continuously obtained during HD treatments by using the Crit-Line monitorTM (CLM). The CLM is used routinely in Renal Research Institute HD units, which allowed us to investigate the ScvO2 in maintenance HD patients. The goals of our study were to evaluate the baseline characteristics of patients with different levels of intradialytic ScvO2 and to examine the associations between ScvO2 and mortality.

Results

Baseline patient characteristics

The final analytical cohort comprised of 232 patients with 6,042 HD treatments and was derived after a deliberate step-by-step data cleaning process at the treatment level. Patients were only excluded in the event that they did not contribute sufficient data during baseline, either because of end of study, death, treatment modality change, recovery of renal function, or transfer to another dialysis facility (Fig. 1).

Figure 1

Study flow chart. HD: hemodialysis, ScvO2: central venous oxygen saturation.

The initial population comprised of 579 patients with CVC as dialysis access, with a total of 15,792 HD treatments with ScvO2 measurements from January 1, 2012 until August 31, 2015. We excluded 3,650 treatments (23%) as they had a mean ScvO2 of greater than 85% and 25 treatments (0.16%) as they had a mean ScvO2 of less than or equal to 25%. We also excluded 4,185 treatments (26.5%) that occurred after the 6-month baseline period. This left us with 579 patients and 7,937 HD sessions, from which we excluded 347 patients with 1,895 HD treatments from the subsequent analysis because they had less than the required 10 HD treatments with ScvO2 recordings and/or less than 6 months of follow up (Fig. 1 ). Out of the 155 patients excluded for not having 6 months of follow up time, 79 were due to death.

In our study population, the mean age was 62.7 ± 15.7 years, dialysis vintage was 2.9 ± 4.6 years, 56% were white, 48.3% were male, 59% had diabetes mellitus (DM), 22% had CHF, and 10.3% had chronic obstructive pulmonary disease (COPD) (Table 1). Median follow-up time was 431 days.

Table 1

Baseline characteristics of all patients, lower tertiles and upper tertile.

Variables	All patients Mean ± SD	Lower Two Tertiles Mean  ± SD	Upper Tertile Mean ± SD	Group Difference Mean (95% CI)	P- value
Patients [N]	232	154	78
Number of eligible HD treatments during baseline [per patient]	26.1 ± 13.3	26.1 ± 13.1	26.1 ± 13.8	0.0 (−3.8 to 3.6)	0.953a
Demographics
Age [years]	62.7 ± 15.7	66.0 ± 13.8	56.2 ± 17.3	9.8 (5.3 to 14.2)	0.001a
Race [% white]	56.0	53.9	60.3	−6.4	0.357b
Gender [% male]	48.3	48.1	48.7	−0.6	0.924b
Vintage [years]	2.9 ± 4.6	3.3 ± 5.1	2.0 ± 3.6	1.3	0.0136c
BMI [kg/m2]	28.1 ± 6.9	28.6 ± 7.0	27.3 ± 6.5	1.2 (−0.7 to 3.2)	0.207a
ScvO 2 saturation [%]
Mean ScvO2	58.7 ± 7.3	54.9 ± 5.3	66.3 ± 4.2	−11.4 (−12.6 to −10.1)	n.a.
Median ScvO2	59.1 ± 7.3	55.2 ± 5.3	66.6 ± 4.2	−11.4 (−12.7 to −10.1)	n.a.
Minimum ScvO2	48.4 ± 9.7	44.4 ± 8.9	56.3 ± 5.7	−11.9 (−13.8 to −10.0)	n.a.
Maximum ScvO2	65.2 ± 6.2	62.0 ± 4.6	71.5 ± 3.6	−9.5 (−10.6 to −8.4)	n.a.
SD ScvO2	3.4 ± 1.1	3.6 ± 1.1	2.9 ± 0.8	0.7 (0.4 to 0.9)	<0.001a
Start ScvO2	59.1 ± 7.4	55.4 ± 5.6	66.5 ± 4.4	−11.1 (−12.4 to −9.8)	n.a.
End ScvO2	57.3 ± 7.8	53.5 ± 6.2	64.8 ± 4.8	−11.3 (−12.7 to −9.8)	n.a.
End – Start ScvO2	−1.8 ± 3.6	−1.9 ± 3.7	−1.7 ± 3.5	−0.2 (−1.2 to 0.8)	0.62
Comorbidities [%]
Diabetes	59.0	60.4	56.4	4.0	0.560b
CHF	22.0	21.4	23.1	−1.7	0.775b
COPD	10.3	11.0	9.0	2.0	0.626b
Treatment parameters
Pre-dialysis SBP [mmHg]	146.4 ± 22.0	143.7 ± 22.9	151.7 ± 19.1	−8.0 (−14.0 to −2.1)	0.009a
Post-dialysis SBP [mmHg]	140.3 ± 20.1	137.8 ± 20.5	145.5 ± 18.2	−7.6 (−13.0 to −2.2)	0.006a
Peridialytic SBP change [mmHg]	−6.1 ± 11.9	−6.0 ± 11.7	−6.4 ± 12.4	0.4 (−2.9 to 3.7)	0.820a
IDWG [kg]	2.0 ± 0.8	1.9 ± 0.8	2.1 ± 0.8	−0.12 (−0.3 to 0.1)	0.249a
IDWG relative to post-dialysis weight [%]	2.6 ± 0.9	2.5 ± 0.9	2.8 ± 1.0	−0.3 (−0.6 to −0.1)	0.007a
UFV [L]	1.9 ± 0.8	1.9 ± 0.79	2.0 ± 0.8	−0.1 (−0.4 to 0.1)	0.173a
Normalized UFV [mL/kg]	25.3 ± 9.7	24 ± 8.9	28 ± 10.7	−4 (−6.6 to −1.4)	0.003a
Post-dialysis weight [kg]	77.4 ± 20.4	79.0 ± 21.4	74.3 ± 18.1	4.6 (−0.9 to 10.2)	0.102a
Treatment time [minutes]	219.0 ± 23	217.7 ± 23.8	221.5 ± 21.1	−3.7 (−10.1 to 2.5)	0.235a
Equilibrated Kt/V	1.5 ± 0.3	1.5 ± 0.3	1.5 ± 0.2	0.0 (−0.1 to 0.1)	0.610a
Laboratory parameters
Hgb [g/dL]	10.6 ± 0.9	10.6 ± 0.9	10.6 ± 0.96	0.0 (−0.3 to 0.3)	0.962a
Serum sodium [mmol/L]	138.6 ± 3.1	138.6 ± 3.2	138.7 ± 2.8	−0.1 (−0.9 to 0.7)	0.782a
Serum potassium [mmol/L]	4.7 ± 0.6	4.6 ± 0.6	4.7 ± 0.4	−0.1 (−0.2 to 0.1)	0.292a
Intact PTH [pg/mL]	518.3 ± 481.1	538.3 ± 498.3	478.6 ± 445.4	59.7 (−72.9 to 192.2)	0.376a
Serum bicarbonate [mmol/L]	23.4 ± 2.2	23.3 ± 2.3	23.7 ± 2.2	−0.4 (−1.0 to 0.2)	0.165a
Leukocytes [1000/µL]	7.0 ± 2.0	7.2 ± 2.1	6.6 ± 1.7	0.6 (0.1 to 1.1)	0.019a
Platelets [1000/µL]	212.9 ± 63.9	216.9 ± 65.1	205.1 ± 61.3	11.8 (−6.5 to 30.1)	0.204a
NLR	4.4 ± 2.6	4.6 ± 2.8	3.8 ± 2.0	0.79 (0.2 to 1.4)	0.015a
Serum albumin [g/dL]	3.8 ± 0.4	3.7 ± 0.4	3.8 ± 0.4	−0.1 (−0.2 to 0.04)	0.165a
Ferritin [ng/mL]	780.5 ± 510.2	798.8 ± 487.9	744.7 ± 552.9	54.1 (−86.8 to 195.0)	0.45a
Transferrin saturation [%]	30.7 ± 9.5	29.8 ± 8.9	32.6 ± 10.5	−2.8 (−5.4 to −0.2)	0.036a

95% CI, 95% confidence interval; SD, standard deviation; ScvO2, central venous oxygen saturation; BMI, body mass index; CHF, congestive heart failure; COPD, chronic obstructive pulmonary disease; SBP, systolic blood pressure; UFV, ultrafiltration volume; IDWG, interdialytic weight gain; Hgb, hemoglobin; PTH, parathyroid hormone; NLR, neutrophil-to-lymphocyte ratio; n.a., not applicable.

a t test.

bChi-square test.

cWilcoxon test.

During baseline, ScvO2 was recorded in 26 ± 13.3 HD treatments per patient. On a population level the ScvO2 was normally distributed with a mean of 58.7 ± 7.3%. Analysis of intradialytic ScvO2 dynamics across all patients indicated that on average ScvO2 slightly increased over the first 60 minutes of treatment, and then progressively declined below starting levels towards the end of HD (Fig. 2).

Figure 2

Time course of mean ScvO2 during hemodialysis in all patients (green), lower two tertiles (red) and upper tertile (blue). The respective 95% confidence intervals are indicated in gray.

ScvO2 as a dichotomous outcomes

Comparison of baseline characteristics between upper and lower tertiles

Patients were stratified into upper tertile (N = 78) and lower two tertiles (N = 154); a mean ScvO2 level below 61.1% during baseline period separated the two groups. A comparison of baseline characteristics between upper and lower tertiles is presented in Table 1. The patients in the lower tertiles were older (66.0 ± 13.8 years vs 56.2 ± 17.3 years, P < 0.001), had longer dialysis vintage (3.3 ± 5.1 years vs 2.0 ± 3.6 years, P = 0.031), lower pre-dialysis systolic blood pressure (SBP) (143.7 ± 22.9 mmHg vs 151.7 ± 19 mmHg, P = 0.009), lower post-dialysis SBP (137.8 ± 20.5 mmHg vs 145.5 ± 18.2 mmHg, P = 0.006), and had lower normalized UFR (6.8 ± 2.4 mL/kg/hr vs 7.7 ± 2.9 mL/kg/hr, P = 0.015). Furthermore, lower tertile subjects had higher leukocyte counts (7.2 ± 2.1 * 1000/µL vs 6.6 ± 1.7 * 1000/µL, P = 0.019) and higher neutrophil-to-lymphocyte ratios (NLR) (4.6 ± 2.8 vs 3.8 ± 2.0, P = 0.015). There was no statistically significant difference in comorbidities of DM, CHF or COPD.

Mortality between upper and lower tertiles

During the 36-month follow-up period, there were a total of 54 deaths, 45 in the lower two tertiles and 9 in the upper tertile. Mortality rate was 24.1/100 patient years in lower two tertiles and 9.0/100 patient years in upper tertile (P = 0.005). Univariate Kaplan-Meier analysis indicated a significantly shorter survival among lower tertile patients (P = 0.0051, log-rank test) (Fig. 3).

Figure 3

Kaplan-Meier estimates for survival probabilities in the lower two tertiles (red) and the upper tertile (blue), respectively. Median follow up for the lower two tertiles was 428 days while the median follow up time for the upper tertile was 432 days. The number of patients at risk is indicated in the table below the graph. The time to death differs significantly between the two groups (P = 0.0051, log-rank test).

ScvO2 as a continuous variable

In unadjusted Cox analysis, for every 1 percent point decrease in mean ScvO2 there was an associated 6% increase in mortality (HR 1.06 (1.03–1.10)). There was no material change in the results after adjustment for age, gender, comorbidities (COPD and CHF), log vintage, inflammatory markers (albumin, NLR), hemoglobin and erythropoietin dose (HR 1.04 (1.01–1.08)) (Table 2 ).

Table 2

Crude and adjusted hazard ratios for all-cause mortality for a 1% decrease in central venous oxygen saturation.

Outcome	Events	Crudea		Minimally Adjustedb		Fully Adjustedc
		HR (95% CI)	P Value	HR (95% CI)	P Value	HR (95% CI)	P Value
All-cause mortality	54	1.06 (1.03 to 1.10)	<0.001	1.05 (1.02 to 1.09)	0.003	1.04 (1.01 to 1.08)	0.0437

HR, hazard ratio.

aUnadjusted model.

bAdjusted for age, gender, chronic obstructive pulmonary disease and congestive heart failure.

cAdjusted for age, gender, chronic obstructive pulmonary disease, congestive heart failure, albumin, hemoglobin, erythropoietin dose, neutrophil to lymphocyte ratio, and log vintage.

Correlates of ScvO2

Figure 4 depicts the relationship between ScvO2 and patient characteristics that were found to differ between the two groups. Mean ScvO2 across patients was plotted against age, log vintage, body mass index (BMI), interdialytic weight gain (IDWG) relative to post-HD weight, post-HD SBP, and NLR. As vintage was not normally distributed, it was log transformed. Age, BMI, log vintage and NLR were negatively associated with ScvO2, while post-HD SBP and IDWG were positively correlated with ScvO2. While all correlates were statistically significant except for ScvO2 and log vintage (P = 0.19), correlation coefficients were relatively low.

Figure 4

Correlates of central venous oxygen saturation with respect to patient characteristics. Each point represents one patient; the depicted data points represent the respective parameter averages during the 6-month baseline period. (A) Age; (B) Log vintage; (C) Body mass index; (D) Interdialytic weight gain relative to post-dialysis body weight; (E) Post-dialysis systolic blood pressure; (F) Neutrophil-to-lymphocyte ratio.

Discussion

Our study indicates that in chronic HD patients with CVC as vascular access, lower ScvO2 levels are associated with poorer survival.

Despite the relative ease with which ScvO2 can be obtained in HD patients with CVC as access, to date only small studies have examined this key indicator of cardiac function. Cordtz et al.[19] in 2008 evaluated 20 HD patients and classified them as either hypotension prone or hypotension resistant and measured their ScvO2 at treatment initiation and end. The authors found a significant decrease in ScvO2 in hypotension prone patients. Harrison et al.[20] investigated 18 HD patients and found a strong inverse correlation between ScvO2 at the end of dialysis and ultrafiltration volume normalized to post-HD body weight. A recent review of intradialytic oxygen saturation did not identify any previous research examining the association between ScvO2 and patient survival[21].

In the study by Harrison et al. the mean ScvO2 was 63.5 ± 13% pre-HD and 56.4 ± 8% post-HD[20], whereas in the study by Cordtz et al. the initial ScvO2 was 52.2 ± 6.7% in hypotension prone and 49.7 ± 6.9% in hypotension resistant patients[19]. While these studies focused on ScvO2 at HD start and end, we examined ScvO2 continuously throughout the HD session. The ScvO2 levels found in our study are below the levels of ~70% observed in healthy subjects[13], but consistent with those reported in HD patients. The exact etiology of low intradialytic ScvO2 in HD patients is not well established, but may be partially explained by the lower hemoglobin levels, and the higher prevalence of cardiac dysfunction and pulmonary hypertension in HD patients.

In our cohort, when ScvO2 was assessed throughout the entire HD treatment, on average ScvO2 increased slightly over the first hour and then progressively declined during the remaining treatment time. The determinants of ScvO2 can be visualized by rearrangement of the familiar form of Fick’s law and replacement of SmvO2 with ScvO2, and CO with upper body blood flow (UBBF), which results in the following equationwith ScvO2 and SaO2 in %, upper body oxygen consumption in mL/min, Hgb in g/L, UBBF in L/min, and K being 1.34, the amount of oxygen (in mL) bound per g of hemoglobin.

Therefore, there are four components which may change during HD and that will cause a decrease in ScvO2; (i) increased tissue oxygen consumption; (ii) a decrease in SaO2, (iii) a decrease in hemoglobin concentration, and (iv) a decrease in upper body blood flow. An increase in oxygen consumption can occur due to an increase in metabolic rate. A small study done in maintenance HD patients found that whole body energy expenditure, measured by indirect calorimetry, increased during HD[22]. However, to what extent the upper body energy expenditure changes during HD is currently unknown. It would be of interest to know if oxygen consumption by the brain, by far the largest consumer of oxygen in the upper body, changes during HD. Intradialytic SaO2 has been demonstrated to decrease during the first hour of HD; unfortunately we do not have SaO2 levels in our patients[23]. While HD patients have lower hemoglobin levels than the general population, during HD as UF occurs and the relative blood volume decreases, hemoglobin levels generally rise due to hemoconcentration[24]. We suspect that a reduction in CO and consequently a decrease in upper body blood flow is the predominant driving factor leading to a drop in ScvO2. The almost linear relation between cerebral perfusion and CO has been recently reviewed[25]. When faced with any of the other possibilities in a patient with intact cardiac function, there should be a compensatory response in CO[26]. There is growing literature on depressed CO during HD treatment due to poor vascular refill and regional wall motion abnormalities (RWMA)[27, 28]. In fact, a recent study using intradialytic magnetic resonance imaging of the heart demonstrated that systolic contractile function fell during HD, with all 12 patients experiencing some degree of segmental left ventricular dysfunction along with evidence of decreased intravascular volume and an inadequate heart rate response[29].

ScvO2 in patients with sepsis, post-surgery, and trauma has been examined, with studies finding that abnormal ScvO2 levels are associated with increased morbidity and mortality[15, 16, 18]. However, the ESRD population is unique in many aspects, and the results of prior studies in other populations therefore may not be fully applicable.

We and others have observed a left shift of the ScvO2 distribution in HD patients compared to healthy subjects, possibly related to anemia and lower CO. Therefore we refrained from defining comparison groups based on ScvO2 levels obtained in healthy subjects but rather used ScvO2 tertiles obtained from our large HD population, where a ScvO2 of 61.1% separated the top from the bottom two tertiles[13, 30]. In a review of literature, ScvO2 levels below 64.4% were associated with morbidity post-surgery, and values below 62% were associated with mortality in patients with pulmonary hypertension[18, 31]. In the trauma setting, ScvO2 < 65% on initial evaluation in the emergency room predicted higher blood loss and greater severity of injuries[16]. We complemented this binary analysis with a continuous spline analysis of the association between ScvO2 and hazard ratio for all-cause mortality; that analysis indicated that mean ScvO2 levels below 63% were associated with increased mortality.

Our finding that patients with lower ScvO2 were older may reflect the poorer cardiac function expected in older subjects. Of note, the prevalence of CHF increases with age, as does CHF mortality[32]. On univariate analysis, age was an independent risk factor for mortality. However, even after adjustment for age in our analysis, ScvO2 as a continuous variable remained a significant predictor of mortality.

While the correlation coefficients were low, we identified several significant correlations between ScvO2 and patient variables such as the association between lower ScvO2 levels and longer dialysis vintage. We speculate that this finding may be related to recurrent hemodynamic stress and cardiac injury caused by HD. McIntyre et al. demonstrated that HD induced RWMA in a subset of maintenance HD patients. While at baseline there was no difference in left ventricular ejection fraction (LVEF) between patients who developed RWMA and those that did not, at 1 year follow-up, the group of patients that developed RWMA during HD had significantly lower resting LVEF[5].

In our study, lower tertile patients had lower pre-HD and lower post-HD SBP, a finding possibly related to low CO. Of note, an association between low pre-HD SBP and mortality has been repeatedly shown[33, 34]. It is interesting to note that in our study the prevalence of CHF did not differ between lower and upper tertiles. Unfortunately, no routine echocardiography assessments were available in our patients, so we cannot comment on the possibility of deficient documentation, classification or misdiagnosis of CHF. One intriguing possibility is that we may be identifying a group of patients without clinically overt signs and symptoms of CHF at rest, who however have reduced cardiac reserve or autonomic dysfunction and are unable to mount the necessary increase in sympathetic response and CO when faced with the hemodynamic stress of HD[27, 35].

The main limitation of our study is its observational nature, which prevents any conclusions related to causality. As mentioned earlier, routine echocardiograms are unfortunately not available in our study population, making potentially very insightful correlational analyses of ScvO2 and cardiac structure and function impossible. Lastly, we appreciate that ScvO2 measurements may be altered by changes in catheter tip position due to changes in body position; however, we have no indication that this may affect one of the two groups disproportionally and created any bias.

Considering a recent review of this topic, we believe that this is the largest study to date examining the epidemiology of ScvO2 in maintenance HD patients[21]. CVC are used as vascular access in the majority of U.S. patients starting HD[1]. While this situation is certainly not desirable, the presence of a CVC allows us to measure ScvO2, a vitally important physiological parameter. This additional diagnostic opportunity may be particularly important in the incident period, the time with the highest cardiovascular morality rate[1]. In fact, a recent study published by Mancini et al. demonstrates that variability in SaO2 is associated with intradialytic hypotension[36]. This supports the potential role of oxygen saturation monitoring during dialysis.

In conclusion, our research shows that routine measurement of ScvO2 during HD provides a novel window into patients’ biology that may help to improve our care for this vulnerable patient population.

Method

Population and study design

This is a retrospective multi-center study of a cohort of maintenance HD patients from 17 facilities of the Renal Research Institute (RRI) across the United States between January 2012 and August 2015. In these clinics, CLM use is part of standard care. All patients were treated with bicarbonate dialysate and polysulfone membranes. Over 80% of patients had a prescribed dialysate temperature of 37 °C. All patients who received HD via a CVC and had at least 6 months of clinical data and 10 dialysis treatments with eligible ScvO2 recordings (definition of eligibility see below) were eligible for inclusion into the study. Therefore our study included both incident and prevalent HD patients. The CLM was rolled out into dialysis units in a staggered manner, and we used the first treatment with CLM data as start date of the patients’ 6 month baseline period. Since eligible patients had to contribute 6 months’ worth of data, by design only those patients who survived for at least 6 months were included into the study (Fig. 1). Patient characteristics were assessed over the baseline period, and mortality was assessed during a follow-up period for a maximum duration of three years. Figure 5 summarizes the study design. For group comparison patients were stratified based on the population ScvO2 that separated the top tertile from the bottom two tertiles. Descriptive statistics of the ScvO2 distribution showed a ScvO2 of 61.1% to be the cut-off between these two groups. Patients were censored in the event of kidney transplantation, transfer to a non-RRI facility, dialysis treatment modality change, recovery of kidney function, or end of follow-up.

Figure 5

Data were reviewed starting from January 1, 2012. Due to the staggered deployment of Crit-line monitors to dialysis units, patients were enrolled into the study on a rolling basis. The first hemodialysis treatment with ScvO2 measurements marked the beginning the 6-month baseline period. Follow-up ended with either end of study (August 31, 2015), death, treatment modality change, recovery of renal function, or transfer to another dialysis facility. ScvO2: central venous oxygen saturation.

The study was approved by the New England Institutional Review Board (14–446) and conducted in accordance with the Declaration of Helsinki. Informed consent was not obtained as this was determined not to be human subject research, and we were working with de-identified data.

This study has been registered at clinicaltrials.gov (NCT02501044).

Measurement of ScvO2

Intradialytic ScvO2 measurements were obtained by the CLM. The CLM has been approved by the U.S. Food and Drug Administration (FDA) for the measurement of hematocrit, relative blood volume, and oxygen saturation in the extracorporeal dialysis circuit. The CLM measures oxygen saturation 9,000 times per minute and reports the mean of these measurements every minute. The manufacturer reported accuracy for oxygen saturation measurement is 2%. Patients’ mean, median, minimum, maximum, standard deviation, start-HD, end-HD ScvO2 was calculated per treatment and then averaged across all treatments per patient and subsequently across patients. We chose to do our analysis using the mean ScvO2 as there was low variability across treatments for each patient (mean coefficient of variability of 7.5 ± 4%).

Clinical and laboratory data

Laboratory measurements were done at Spectra East Laboratories (Rockleigh, NJ, USA). The results were downloaded to the RRI data warehouse and extracted to the study database. Continuous variables were averaged during the baseline period. BMI was calculated using post-HD dry weight.

Data eligibility

To ensure appropriate data quality, we included only treatments where mean ScvO2 was below 85%, as higher values are incompatible with central venous blood[13]. Mean ScvO2 measurements less than 25% were excluded because they are considered incompatible with life[37]. Additionally, data points with relative blood volume measurements above 102% were considered very unlikely, potentially due to saline administration, and hence excluded. This constituted 3% of all data points.

Comorbidities

CHF, DM, and COPD were defined using International Classification of Diseases - 9 (ICD-9) codes.

Statistical analysis

Continuous variables are presented as mean ± standard deviation (SD) if normally distributed and as median (25th, 75th percentile) otherwise. Categorical variables are presented as percentages of the respective group. Statistics of ScvO2 variables were calculated on a HD treatment level and then aggregated on a patient level.

Baseline characteristics of exposed and unexposed were compared using chi-square test for categorical variables and two-sample t test for continuous variables, Wilcoxon Rank-Sum test were used for non-parametric variables. Survival characteristics were compared using Kaplan-Meier plots, log-rank test, and Cox proportional hazards models.

Statistical analyses were performed using SAS version 9.3 (SAS Institute Inc., Cary, NC) and R 3.0.2 (libraries ggplot2, splines, survival, pspline; R Foundation for Statistical Computing, Vienna, Austria).

Disclosure

P.K. holds stock in Fresenius Medical Care. L.C. is supported in part by the NIH (5T32DK007757-18). The other authors declared no competing interest. The results presented in this paper have not been published previously in whole or part, except in abstract format.

Data Availability

Consolidated data may be shared with other scientists at their request.