Pilar Eguía-Aguilar1, Lisette Gutiérrez-Castillo1,2, Mario Pérezpeña-Díazconti1, Jeanette García-Chéquer1, Jorge García-Quintana1, Fernando Chico-Ponce de León3, Luis Gordillo-Domínguez3, Samuel Torres-García3, Francisco Arenas-Huertero4,5. 1. Departmento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, México. 2. Facultad de Ciencia y Tecnología, Universidad Simón Bolívar, Ciudad de México, México. 3. Departmento de Neurocirugía, Hospital Infantil de México Federico Gómez, Ciudad de México, México. 4. Facultad de Ciencia y Tecnología, Universidad Simón Bolívar, Ciudad de México, México. farenashuertero@yahoo.com.mx. 5. Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez-Instituto Nacional de Salud, Dr. Márquez 162, Colonia Doctores, Delegación Cuauhtémoc, 06720, Ciudad de México, México. farenashuertero@yahoo.com.mx.
Abstract
PURPOSE: MicroRNAs were identified as molecules that participate in gene regulation; alterations in their expression characterize central nervous system (CNS). Information in pediatrics is scarce, so the objective of this work was to determine and then compare the patterns of expression of microRNAs in astrocytomas, ependymomas, and medulloblastomas, as well as in non-neoplastic brain. METHODS: Low-density arrays were utilized to evaluate 756 microRNAs in three samples of each type of tumor and non-neoplastic brain. The relative expression was calculated in order to identify the three microRNAs whose expression was modified notably. This was verified using RT-qPCR in more number of tumor samples. RESULTS: The microRNAs selected for testing were miR-100-5p, miR-195-5p, and miR-770-5p. A higher expression of miR-100-5p was observed in the astrocytomas and ependymomas compared to the medulloblastomas: on average 3.8 times (p < 0.05). MiR-770-5p was expressed less in medulloblastomas compared to astrocytomas four times (p = 0.0162). MiR-195-5p had a low expression in medulloblastomas compared to non-neoplastic cerebellum (p = 0.049). In all three tumor types, expression of miR-770-5p was lower than in non-neoplastic brain (p < 0.001). CONCLUSIONS: These microRNAs may represent potential markers in these tumors.
PURPOSE: MicroRNAs were identified as molecules that participate in gene regulation; alterations in their expression characterize central nervous system (CNS). Information in pediatrics is scarce, so the objective of this work was to determine and then compare the patterns of expression of microRNAs in astrocytomas, ependymomas, and medulloblastomas, as well as in non-neoplastic brain. METHODS: Low-density arrays were utilized to evaluate 756 microRNAs in three samples of each type of tumor and non-neoplastic brain. The relative expression was calculated in order to identify the three microRNAs whose expression was modified notably. This was verified using RT-qPCR in more number of tumor samples. RESULTS: The microRNAs selected for testing were miR-100-5p, miR-195-5p, and miR-770-5p. A higher expression of miR-100-5p was observed in the astrocytomas and ependymomas compared to the medulloblastomas: on average 3.8 times (p < 0.05). MiR-770-5p was expressed less in medulloblastomas compared to astrocytomas four times (p = 0.0162). MiR-195-5p had a low expression in medulloblastomas compared to non-neoplastic cerebellum (p = 0.049). In all three tumor types, expression of miR-770-5p was lower than in non-neoplastic brain (p < 0.001). CONCLUSIONS: These microRNAs may represent potential markers in these tumors.
Authors: William A Freije; F Edmundo Castro-Vargas; Zixing Fang; Steve Horvath; Timothy Cloughesy; Linda M Liau; Paul S Mischel; Stanley F Nelson Journal: Cancer Res Date: 2004-09-15 Impact factor: 12.701
Authors: S A Ciafrè; S Galardi; A Mangiola; M Ferracin; C-G Liu; G Sabatino; M Negrini; G Maira; C M Croce; M G Farace Journal: Biochem Biophys Res Commun Date: 2005-09-09 Impact factor: 3.575
Authors: Mamoun Ahram; Justin Z Amarin; Haya H Suradi; Sultan S Abdelhamid; Mona M Makhamreh; Randa M Bawadi; Maysa Al-Hussaini Journal: J Mol Neurosci Date: 2018-09-25 Impact factor: 3.444