Literature DB >> 28811205

Extraction and characterization of acid-soluble and pepsin-soluble collagen from skin of loach (Misgurnus anguillicaudatus).

Jing Wang1, Xinli Pei2, Haiying Liu3, Dan Zhou4.   

Abstract

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from loach skin. The yields of ASC and PSC were 22.42% and 27.32%, respectively. Sodium dodecyl sulfate-polyacrylamide-gel electrophoresis and peptide mass fingerprint analysis revealed that loach skin contained type I collagen. There were 212 imino acids/1000 residues in ASC and 193 imino acids/1000 residues in PSC. Fourier transform infrared spectrometry analysis, UV measurements and circular dichroism confirmed that loach skin collagen had a triple helical structure. The denaturation temperatures of ASC and PSC were 36.03°C and 33.61°C, respectively. Zeta potential analysis revealed that net zero charge values of ASC and PSC were 6.42 and 6.51, respectively. Therefore, loach skin collagen may be an alternative to terrestrial mammalian collagen and may enhance the added value of this fish species.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Characterization; Circular dichroism; Collagen; Loach skin; Peptide mass fingerprint

Mesh:

Substances:

Year:  2017        PMID: 28811205     DOI: 10.1016/j.ijbiomac.2017.08.046

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN: 0141-8130            Impact factor:   6.953


  12 in total

1.  Physicochemical properties of acid- and pepsin-soluble collagens from the cartilage of Siberian sturgeon.

Authors:  Qian-Bin Luo; Chang-Feng Chi; Fan Yang; Yu-Qin Zhao; Bin Wang
Journal:  Environ Sci Pollut Res Int       Date:  2018-09-08       Impact factor: 4.223

2.  Microstructural and Physicochemical Analysis of Collagens from the Skin of Lizardfish (Saurida tumbil Bloch, 1795) Extracted with Different Organic Acids.

Authors:  Abdul Aziz Jaziri; Rossita Shapawi; Ruzaidi Azli Mohd Mokhtar; Wan Norhana Md Noordin; Nurul Huda
Journal:  Molecules       Date:  2022-04-11       Impact factor: 4.927

Review 3.  Hydrolyzed Collagen-Sources and Applications.

Authors:  Arely León-López; Alejandro Morales-Peñaloza; Víctor Manuel Martínez-Juárez; Apolonio Vargas-Torres; Dimitrios I Zeugolis; Gabriel Aguirre-Álvarez
Journal:  Molecules       Date:  2019-11-07       Impact factor: 4.411

4.  Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin.

Authors:  Junde Chen; Jianying Li; Zhongbao Li; Ruizao Yi; Shenjia Shi; Kunyuan Wu; Yushuang Li; Sijia Wu
Journal:  Mar Drugs       Date:  2019-09-28       Impact factor: 5.118

5.  Therapeutic Effect of Nile Tilapia Type II Collagen on Rigidity in CD8+ Cells by Alleviating Inflammation and Rheumatoid Arthritis in Rats by Oral Tolerance.

Authors:  Chunyu Hou; Na Li; Mengyao Liu; Jingjing Chen; Jeevithan Elango; Saeed Ur Rahman; Bin Bao; Wenhui Wu
Journal:  Polymers (Basel)       Date:  2022-03-22       Impact factor: 4.329

6.  Preparation, Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Swim Bladders of Miiuy Croaker (Miichthys miiuy).

Authors:  Wen-Hao Zhao; Chang-Feng Chi; Yu-Qin Zhao; Bin Wang
Journal:  Mar Drugs       Date:  2018-05-12       Impact factor: 5.118

7.  Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings.

Authors:  Baosheng Ge; Haonan Wang; Jie Li; Hengheng Liu; Yonghao Yin; Naili Zhang; Song Qin
Journal:  Mar Drugs       Date:  2020-03-25       Impact factor: 5.118

8.  Physicochemical Properties and Biocompatibility Evaluation of Collagen from the Skin of Giant Croaker (Nibea japonica).

Authors:  Yunping Tang; Shujie Jin; Xiaoyan Li; Xiaojuan Li; Xuyang Hu; Yan Chen; Fangfang Huang; Zuisu Yang; Fangmiao Yu; Guofang Ding
Journal:  Mar Drugs       Date:  2018-06-29       Impact factor: 5.118

9.  Collagen Extracted from Bigeye Tuna (Thunnus obesus) Skin by Isoelectric Precipitation: Physicochemical Properties, Proliferation, and Migration Activities.

Authors:  Xinhui Lin; Yinyue Chen; Huoxi Jin; Qiaoling Zhao; Chenjuan Liu; Renwei Li; Fangmiao Yu; Yan Chen; Fangfang Huang; Zuisu Yang; Guofang Ding; Yunping Tang
Journal:  Mar Drugs       Date:  2019-05-01       Impact factor: 5.118

10.  Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Scales of Miiuy Croaker (Miichthys Miiuy).

Authors:  Long-Yan Li; Yu-Qin Zhao; Yu He; Chang-Feng Chi; Bin Wang
Journal:  Mar Drugs       Date:  2018-10-20       Impact factor: 5.118

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.