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Literature DB >> 28798043

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9.

Dong Niu^1,2, Hong-Jiang Wei^3,4, Lin Lin⁵, Haydy George¹, Tao Wang¹, I-Hsiu Lee¹, Hong-Ye Zhao³, Yong Wang⁶, Yinan Kan¹, Ellen Shrock⁷, Emal Lesha¹, Gang Wang¹, Yonglun Luo⁵, Yubo Qing^3,4, Deling Jiao^3,4, Heng Zhao^3,4, Xiaoyang Zhou⁶, Shouqi Wang⁸, Hong Wei⁶, Marc Güell¹, George M Church^1,7,9, Luhan Yang¹⁰.

Abstract

Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizontal transfer of PERVs among human cells. Using CRISPR-Cas9, we inactivated all of the PERVs in a porcine primary cell line and generated PERV-inactivated pigs via somatic cell nuclear transfer. Our study highlights the value of PERV inactivation to prevent cross-species viral transmission and demonstrates the successful production of PERV-inactivated animals to address the safety concern in clinical xenotransplantation.

Entities: CellLine Chemical Disease Gene Mutation Species

Mesh：

Year: 2017 PMID： 28798043 PMCID： PMC5813284 DOI： 10.1126/science.aan4187

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

11 in total

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Authors: Jack-Yves Deschamps; Françoise A Roux; Pierre Saï; Edouard Gouin
Journal: Xenotransplantation Date: 2005-03 Impact factor: 3.907

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3. Organ shortage: the greatest challenge facing transplant medicine.

Authors: David Shafran; Eric Kodish; Andreas Tzakis
Journal: World J Surg Date: 2014-07 Impact factor: 3.352

4. Infection of human cells by an endogenous retrovirus of pigs.

Authors: C Patience; Y Takeuchi; R A Weiss
Journal: Nat Med Date: 1997-03 Impact factor: 53.440

5. Multiple groups of novel retroviral genomes in pigs and related species.

Authors: C Patience; W M Switzer; Y Takeuchi; D J Griffiths; M E Goward; W Heneine; J P Stoye; R A Weiss
Journal: J Virol Date: 2001-03 Impact factor: 5.103

Review 6. Effect of epigenetic regulation during swine embryogenesis and on cloning by nuclear transfer.

Authors: Jianguo Zhao; Jeffrey Whyte; Randall S Prather
Journal: Cell Tissue Res Date: 2010-06-20 Impact factor: 5.249

7. Porcine endogenous retrovirus integration sites in the human genome: features in common with those of murine leukemia virus.

Authors: Yann Moalic; Yannick Blanchard; Hélène Félix; André Jestin
Journal: J Virol Date: 2006-08-23 Impact factor: 5.103

8. Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

Authors: Luhan Yang; Marc Güell; Dong Niu; Haydy George; Emal Lesha; Dennis Grishin; John Aach; Ellen Shrock; Weihong Xu; Jürgen Poci; Rebeca Cortazio; Robert A Wilkinson; Jay A Fishman; George Church
Journal: Science Date: 2015-10-11 Impact factor: 47.728

9. Analyses of pig genomes provide insight into porcine demography and evolution.

Authors: Martien A M Groenen; Alan L Archibald; Hirohide Uenishi; Christopher K Tuggle; Yasuhiro Takeuchi; Max F Rothschild; Claire Rogel-Gaillard; Chankyu Park; Denis Milan; Hendrik-Jan Megens; Shengting Li; Denis M Larkin; Heebal Kim; Laurent A F Frantz; Mario Caccamo; Hyeonju Ahn; Bronwen L Aken; Anna Anselmo; Christian Anthon; Loretta Auvil; Bouabid Badaoui; Craig W Beattie; Christian Bendixen; Daniel Berman; Frank Blecha; Jonas Blomberg; Lars Bolund; Mirte Bosse; Sara Botti; Zhan Bujie; Megan Bystrom; Boris Capitanu; Denise Carvalho-Silva; Patrick Chardon; Celine Chen; Ryan Cheng; Sang-Haeng Choi; William Chow; Richard C Clark; Christopher Clee; Richard P M A Crooijmans; Harry D Dawson; Patrice Dehais; Fioravante De Sapio; Bert Dibbits; Nizar Drou; Zhi-Qiang Du; Kellye Eversole; João Fadista; Susan Fairley; Thomas Faraut; Geoffrey J Faulkner; Katie E Fowler; Merete Fredholm; Eric Fritz; James G R Gilbert; Elisabetta Giuffra; Jan Gorodkin; Darren K Griffin; Jennifer L Harrow; Alexander Hayward; Kerstin Howe; Zhi-Liang Hu; Sean J Humphray; Toby Hunt; Henrik Hornshøj; Jin-Tae Jeon; Patric Jern; Matthew Jones; Jerzy Jurka; Hiroyuki Kanamori; Ronan Kapetanovic; Jaebum Kim; Jae-Hwan Kim; Kyu-Won Kim; Tae-Hun Kim; Greger Larson; Kyooyeol Lee; Kyung-Tai Lee; Richard Leggett; Harris A Lewin; Yingrui Li; Wansheng Liu; Jane E Loveland; Yao Lu; Joan K Lunney; Jian Ma; Ole Madsen; Katherine Mann; Lucy Matthews; Stuart McLaren; Takeya Morozumi; Michael P Murtaugh; Jitendra Narayan; Dinh Truong Nguyen; Peixiang Ni; Song-Jung Oh; Suneel Onteru; Frank Panitz; Eung-Woo Park; Hong-Seog Park; Geraldine Pascal; Yogesh Paudel; Miguel Perez-Enciso; Ricardo Ramirez-Gonzalez; James M Reecy; Sandra Rodriguez-Zas; Gary A Rohrer; Lauretta Rund; Yongming Sang; Kyle Schachtschneider; Joshua G Schraiber; John Schwartz; Linda Scobie; Carol Scott; Stephen Searle; Bertrand Servin; Bruce R Southey; Goran Sperber; Peter Stadler; Jonathan V Sweedler; Hakim Tafer; Bo Thomsen; Rashmi Wali; Jian Wang; Jun Wang; Simon White; Xun Xu; Martine Yerle; Guojie Zhang; Jianguo Zhang; Jie Zhang; Shuhong Zhao; Jane Rogers; Carol Churcher; Lawrence B Schook
Journal: Nature Date: 2012-11-15 Impact factor: 49.962

Review 10. How Active Are Porcine Endogenous Retroviruses (PERVs)?

Authors: Joachim Denner
Journal: Viruses Date: 2016-08-03 Impact factor: 5.048

171 in total

1. An effective vaginal gel to deliver CRISPR/Cas9 system encapsulated in poly (β-amino ester) nanoparticles for vaginal gene therapy.

Authors: Gang Niu; Zhuang Jin; Chong Zhang; Dan He; Xueqin Gao; Chenming Zou; Wei Zhang; Jiahui Ding; Bhudev C Das; Konstantin Severinov; Inga Isabel Hitzeroth; Priya Ranjan Debata; Xin Ma; Xun Tian; Qinglei Gao; Jun Wu; Zeshan You; Rui Tian; Zifeng Cui; Weiwen Fan; Weiling Xie; Zhaoyue Huang; Chen Cao; Wei Xu; Hongxian Xie; Hongyan Xu; Xiongzhi Tang; Yan Wang; Zhiying Yu; Hui Han; Songwei Tan; Shuqin Chen; Zheng Hu
Journal: EBioMedicine Date: 2020-07-22 Impact factor: 8.143

Review 2. The origin of porcine endogenous retroviruses (PERVs).

Authors: Joachim Denner
Journal: Arch Virol Date: 2021-02-06 Impact factor: 2.574

3. The ethics of genome editing in non-human animals: a systematic review of reasons reported in the academic literature.

Authors: Nienke de Graeff; Karin R Jongsma; Josephine Johnston; Sarah Hartley; Annelien L Bredenoord
Journal: Philos Trans R Soc Lond B Biol Sci Date: 2019-05-13 Impact factor: 6.237

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9.

1. History of xenotransplantation.

Review 2. Retrovirus-induced acquired immunodeficiencies.

3. Organ shortage: the greatest challenge facing transplant medicine.

4. Infection of human cells by an endogenous retrovirus of pigs.

5. Multiple groups of novel retroviral genomes in pigs and related species.

Review 6. Effect of epigenetic regulation during swine embryogenesis and on cloning by nuclear transfer.

7. Porcine endogenous retrovirus integration sites in the human genome: features in common with those of murine leukemia virus.

8. Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

9. Analyses of pig genomes provide insight into porcine demography and evolution.

Review 10. How Active Are Porcine Endogenous Retroviruses (PERVs)?

1. An effective vaginal gel to deliver CRISPR/Cas9 system encapsulated in poly (β-amino ester) nanoparticles for vaginal gene therapy.

Review 2. The origin of porcine endogenous retroviruses (PERVs).

3. The ethics of genome editing in non-human animals: a systematic review of reasons reported in the academic literature.

4. Kidney Xenotransplantation: Steps toward Clinical Application.

5. CRISPR, animals, and FDA oversight: Building a path to success.

Review 6. Advances in the immunology of heart transplantation.

Review 7. Nanotechnology in cell replacement therapies for type 1 diabetes.

Review 8. Public health applications of CRISPR: How children's health can benefit.

9. Kidney Regeneration in Later-Stage Mouse Embryos via Transplanted Renal Progenitor Cells.

Review 10. Can We Re-Engineer the Endocrine Pancreas?