Uta Jappe1,2, Christian Schwager3. 1. Division of Clinical and Molecular Allergology, Priority Research Area Asthma & Allergy, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Research Center Borstel, Parkallee 35, 23845, Borstel, Germany. ujappe@fz-borstel.de. 2. Interdisciplinary Allergy Outpatient Clinic, Department of Pneumology, University of Lübeck, Lübeck, Germany. ujappe@fz-borstel.de. 3. Division of Clinical and Molecular Allergology, Priority Research Area Asthma & Allergy, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Research Center Borstel, Parkallee 35, 23845, Borstel, Germany.
Abstract
PURPOSE OF REVIEW: The purpose of this review is to provide available data on a new class of allergens, the oleosins, and their diagnostic value. There is evidence that allergen extracts used for in vivo as well as in vitro diagnostic tests do not contain oleosins because these proteins are lipophilic and nearly insoluble in saline or aqueous solutions. So far, only oleosins of peanut, sesame and hazelnut have been registered as allergens. Reports on IgE-binding tests performed with oleosins of different species with sera from allergic patients show that IgE specific for oleosins are associated with severe allergic reactions which is why they should be part of the diagnostic tests in the future. RECENT FINDINGS: Recent findings showed that oleosins purified from in shell-roasted peanuts revealed a higher IgE-binding capacity when compared to raw ones. Naturally purified as well as recombinantly produced peanut oleosins can be used in basophil activation test. The synopsis of all reports on different thermal processing of several oleosin sources and the respective data obtained with patients sera investigated via immunoblot and basophil activation test points to the recommendation that-if naturally purified oleosins are used, they should mostly be obtained from roasted food allergen sources. For immunoblot and basophil activation test, both, naturally purified oleosins as well as recombinant modified oleosins are valuable diagnostic tools.
PURPOSE OF REVIEW: The purpose of this review is to provide available data on a new class of allergens, the oleosins, and their diagnostic value. There is evidence that allergen extracts used for in vivo as well as in vitro diagnostic tests do not contain oleosins because these proteins are lipophilic and nearly insoluble in saline or aqueous solutions. So far, only oleosins of peanut, sesame and hazelnut have been registered as allergens. Reports on IgE-binding tests performed with oleosins of different species with sera from allergicpatients show that IgE specific for oleosins are associated with severe allergic reactions which is why they should be part of the diagnostic tests in the future. RECENT FINDINGS: Recent findings showed that oleosins purified from in shell-roasted peanuts revealed a higher IgE-binding capacity when compared to raw ones. Naturally purified as well as recombinantly produced peanutoleosins can be used in basophil activation test. The synopsis of all reports on different thermal processing of several oleosin sources and the respective data obtained with patients sera investigated via immunoblot and basophil activation test points to the recommendation that-if naturally purified oleosins are used, they should mostly be obtained from roasted food allergen sources. For immunoblot and basophil activation test, both, naturally purified oleosins as well as recombinant modified oleosins are valuable diagnostic tools.
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