Matheus Levi Tajra Feitosa1, Carlos Alberto Palmeira Sarmento1, Renato Zonzini Bocabello2, Patrícia Cristina Baleeiro Beltrão-Braga3, Graciela Conceição Pignatari4, Robson Fortes Giglio5, Maria Angelica Miglino6, Jéssica Rodrigues Orlandin7, Carlos Eduardo Ambrósio8. 1. Fellow PhD degree, Postgraduate Program in Anatomy in Domestic and Wild Animals, Department of Surgery, Faculty of Veterinary Medicine and Animal Science, Universidade de São Paulo (USP), Brazil. Intellectual and scientific content of the study; conception and design of the study; acquisition, analysis and interpretation of data, manuscript preparation. 2. Fellow Master degree, Postgraduate Program in Anatomy in Domestic and Wild Animals, Department of Surgery, Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study; conception and design of the study; acquisition, analysis and interpretation of data; manuscript preparation. 3. Assistant Professor, Department of Surgery, Stem Cell Laboratory, USP, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study, acquisition of human stem cells, analysis and interpretation of data, manuscript writing. 4. PosDoc Fellow, Department of Surgery, Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study, acquisition of human stem cells, analysis and interpretation of data, manuscript writing. 5. Assistant Professor, Department of Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA. Intellectual and scientific content of the study; conception and design of the study; acquisition, analysis and interpretation of data; manuscript preparation and writing. 6. Researcher, CNPq Grant Level 1A - CA VT, Professor, Chairwoman, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, USP, Pirassunung-SP, Brazil. Conception and design of the study, manuscript writing, critical revision, final approval. 7. Fellow Master degree, Postgraduate Program in Animal Bioscience, Laboratory of Stem Cell and Gene Therapy, Veterinary Medicine Department, Faculty of Animal Science and Food Engineering, USP, Pirassununga-SP, Brazil. Manuscript preparation. 8. Researcher, CNPq Grant Level 1A - CA VT, Associate Professor III, Head, Department Veterinary Medicine, Laboratory of Stem Cell and Gene Therapy, Veterinary Medicine Department, Faculty of Animal Science and Food Engineering, USP, Pirassununga-SP, Brazil. Conception and design of the study, manuscript writing, critical revision, final approval.
Abstract
PURPOSE: : To investigate the therapeutic potential of human immature dental pulp stem cells in the treatment of chronic spinal cord injury in dogs. METHODS: : Three dogs of different breeds with chronic SCI were presented as animal clinical cases. Human immature dental pulp stem cells were injected at three points into the spinal cord, and the animals were evaluated by limb function and magnetic resonance imaging (MRI) pre and post-operative. RESULTS: : There was significant improvement from the limb function evaluated by Olby Scale, though it was not supported by the imaging data provided by MRI and clinical sign and evaluation. CONCLUSION: : Human dental pulp stem cell therapy presents promising clinical results in dogs with chronic spinal cord injuries, if used in association with physical therapy.
PURPOSE: : To investigate the therapeutic potential of human immature dental pulp stem cells in the treatment of chronic spinal cord injury in dogs. METHODS: : Three dogs of different breeds with chronic SCI were presented as animal clinical cases. Human immature dental pulp stem cells were injected at three points into the spinal cord, and the animals were evaluated by limb function and magnetic resonance imaging (MRI) pre and post-operative. RESULTS: : There was significant improvement from the limb function evaluated by Olby Scale, though it was not supported by the imaging data provided by MRI and clinical sign and evaluation. CONCLUSION: : Human dental pulp stem cell therapy presents promising clinical results in dogs with chronic spinal cord injuries, if used in association with physical therapy.
Authors: Naira Caroline Godoy Pieri; Aline Fernanda de Souza; Ramon Cesar Botigelli; Lucas Simões Machado; Carlos Eduardo Ambrosio; Daniele Dos Santos Martins; André Furugen Cesar de Andrade; Flavio Vieira Meirelles; Poul Hyttel; Fabiana Fernandes Bressan Journal: Vet Res Commun Date: 2019-01-17 Impact factor: 2.459
Authors: Jéssica Rodrigues Orlandin; Ingrid da Silva Gomes; Shamira de Fátima Sallum Leandro; Artur Fuertes Cagnim; Juliana Barbosa Casals; Adriano Bonfim Carregaro; Silvio Henrique Freitas; Luciana Cristina Machado; Maria Cristina Reis Castiglioni; Ana Liz Garcia Alves; Vânia Maria de Vasconcelos Machado; Carlos Eduardo Ambrósio Journal: Stem Cells Cloning Date: 2021-10-18
Authors: Fernando Swiech Bach; Carmen Lucia Kuniyoshi Rebelatto; Leticia Fracaro; Alexandra Cristina Senegaglia; Felipe Yukio Ishikawa Fragoso; Debora Regina Daga; Paulo Roberto Slud Brofman; Claudia Turra Pimpão; Jair Rodini Engracia Filho; Fabiano Montiani-Ferreira; José Ademar Villanova Journal: Front Vet Sci Date: 2019-11-08