Maryam Pourhajibagher1,2,3, Hosein Kazemian1, Nasim Chiniforush2, Abbas Bahador1,2,3. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Laser Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND AND AIMS: Pandrug-resistant Acinetobacter baumannii (PDRAB) are including colistin resistant starins (CoRAB) which cause infections potentially untreatable infections. Recently, incidence of these strains are increasing worldwide. Therefore, new approaches, methods and strategies are urgently needed for treatment and eradication of infections due to PDRAB. So the aim of this study was to evaluate the efficacy of photodynamic therapy (PDT) in combination treatment with colistin against PDRAB. MATERIALS AND METHODS: PDRAB which was isolated from burn patients was used as a test strain. PDT carried out in which toluidine blue O (TBO) and light-emitting diode (LED) were used as photosensitizer and radiation source, respectively. Then, the effect of PDT plus colistin was evaluated on CoRAB and the colony-forming units of each tested groups calculated. Finally, confirmation of antibacterial activity of combination therapy was carried out using scanning electron microscope. RESULTS: PDT declined bacterial count in comparing with control group by 83.7% of killing percentage, in other words, less than one log reduction. While PDT in combination with colistin showed high synergetic effect against A. baumannii in all concentrations of colistin tested by 100% of killing percentage with 9-log reduction. CONCLUSIONS: According to our results, PDT alone couldn't eliminate all of the treated bacterial cells. But when combined with colistin, it killed all of the treated bacterial cells in all tested concentrations. Also PDT decreased the minimal inhibitory concentration of colistin against PDRAB by more than 11 fold.
BACKGROUND AND AIMS: Pandrug-resistant Acinetobacter baumannii (PDRAB) are including colistin resistant starins (CoRAB) which cause infections potentially untreatable infections. Recently, incidence of these strains are increasing worldwide. Therefore, new approaches, methods and strategies are urgently needed for treatment and eradication of infections due to PDRAB. So the aim of this study was to evaluate the efficacy of photodynamic therapy (PDT) in combination treatment with colistin against PDRAB. MATERIALS AND METHODS: PDRAB which was isolated from burn patients was used as a test strain. PDT carried out in which toluidine blue O (TBO) and light-emitting diode (LED) were used as photosensitizer and radiation source, respectively. Then, the effect of PDT plus colistin was evaluated on CoRAB and the colony-forming units of each tested groups calculated. Finally, confirmation of antibacterial activity of combination therapy was carried out using scanning electron microscope. RESULTS: PDT declined bacterial count in comparing with control group by 83.7% of killing percentage, in other words, less than one log reduction. While PDT in combination with colistin showed high synergetic effect against A. baumannii in all concentrations of colistin tested by 100% of killing percentage with 9-log reduction. CONCLUSIONS: According to our results, PDT alone couldn't eliminate all of the treated bacterial cells. But when combined with colistin, it killed all of the treated bacterial cells in all tested concentrations. Also PDT decreased the minimal inhibitory concentration of colistin against PDRAB by more than 11 fold.
Authors: Lona Mody; Kristen E Gibson; Amanda Horcher; Katherine Prenovost; Sara E McNamara; Betsy Foxman; Keith S Kaye; Suzanne Bradley Journal: Infect Control Hosp Epidemiol Date: 2015-06-15 Impact factor: 3.254
Authors: Joana Almeida; João P C Tomé; Maria G P M S Neves; Augusto C Tomé; José A S Cavaleiro; Ângela Cunha; Liliana Costa; Maria A F Faustino; Adelaide Almeida Journal: Photochem Photobiol Sci Date: 2014-04 Impact factor: 3.982