H-Y Hong1,2, F-H Chen1, Y-Q Sun1, X-T Hu3, Y Wei1, Y-P Fan2, J Zhang1, D-H Wang3, R Xu1, H-B Li3,4, J-B Shi1. 1. Allergy Center, Otorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. 2. Department of Otolaryngology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China. 3. Department of Otolaryngology, Head and Neck Surgery, Affiliated Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China. 4. Department of Otolaryngology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Abstract
BACKGROUND: IL-25 has been proposed to play a key role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to evaluate the association of IL-25 with the Th2-biased inflammatory profiles in CRSwNP. METHODS: Nasal polyp (NP) tissues and control uncinate process tissues were collected from 92 patients with CRSwNP, 20 patients with chronic rhinosinusitis without nasal polyps (CRSsNP), and 16 normal control subjects. IL-25 expression was examined using immunohistochemistry and immunofluorescence staining, flow cytometry, RT-qPCR, and ELISA. The inflammatory profiles and clinical characteristics of 2 NP subtypes (IL-25high and IL-25low ) were evaluated, and the effects of IL-25 on Th2 cytokine production in cultured dispersed polyp cells were examined in vitro. RESULTS: The mRNA and protein levels of IL-25 were significantly increased in the polyp tissues compared with the control uncinate process tissues. The IL-25high subtype showed greater computed tomography scores, endoscopic scores, and Th2 response. Exposure to IL-25 activated type 2 innate lymphoid cells and Th2 cells in NP simultaneously which further increased Th2 cytokine production in vitro. CONCLUSIONS: Local IL-25 plays a crucial role in promoting Th2-biased inflammatory profiles in NP and may serve as a promising therapeutic target in CRSwNP patients.
BACKGROUND:IL-25 has been proposed to play a key role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to evaluate the association of IL-25 with the Th2-biased inflammatory profiles in CRSwNP. METHODS: Nasal polyp (NP) tissues and control uncinate process tissues were collected from 92 patients with CRSwNP, 20 patients with chronic rhinosinusitis without nasal polyps (CRSsNP), and 16 normal control subjects. IL-25 expression was examined using immunohistochemistry and immunofluorescence staining, flow cytometry, RT-qPCR, and ELISA. The inflammatory profiles and clinical characteristics of 2 NP subtypes (IL-25high and IL-25low ) were evaluated, and the effects of IL-25 on Th2 cytokine production in cultured dispersed polyp cells were examined in vitro. RESULTS: The mRNA and protein levels of IL-25 were significantly increased in the polyp tissues compared with the control uncinate process tissues. The IL-25high subtype showed greater computed tomography scores, endoscopic scores, and Th2 response. Exposure to IL-25 activated type 2 innate lymphoid cells and Th2 cells in NP simultaneously which further increased Th2 cytokine production in vitro. CONCLUSIONS: Local IL-25 plays a crucial role in promoting Th2-biased inflammatory profiles in NP and may serve as a promising therapeutic target in CRSwNP patients.
Authors: Neil N Patel; Vasiliki Triantafillou; Ivy W Maina; Alan D Workman; Charles C L Tong; Edward C Kuan; Peter Papagiannopoulos; John V Bosso; Nithin D Adappa; James N Palmer; Michael A Kohanski; De'Broski R Herbert; Noam A Cohen Journal: Int Forum Allergy Rhinol Date: 2019-03-20 Impact factor: 3.858
Authors: Neil N Patel; Michael A Kohanski; Ivy W Maina; Alan D Workman; De'Broski R Herbert; Noam A Cohen Journal: Int Forum Allergy Rhinol Date: 2018-09-10 Impact factor: 3.858
Authors: Neil N Patel; Michael A Kohanski; Ivy W Maina; Vasiliki Triantafillou; Alan D Workman; Charles C L Tong; Edward C Kuan; John V Bosso; Nithin D Adappa; James N Palmer; De'Broski R Herbert; Noam A Cohen Journal: Int Forum Allergy Rhinol Date: 2018-05-09 Impact factor: 3.858
Authors: Haiyu Hong; Kai Sen Tan; Yan Yan; Fenghong Chen; Hsiao Hui Ong; Yukei Oo; Jing Liu; Yew Kwang Ong; Mark Thong; Richard Sugrue; Vincent T Chow; De Yun Wang Journal: J Inflamm Res Date: 2021-06-28