A Gram-Scale HPLC-Free Synthesis of TNA Triphosphates Using an Iterative Phosphorylation Strategy.

α-l-Threofuranosyl nucleoside 3'-triphosphates (tNTPs) bearing the four genetic bases adenine (A), cytosine (C), guanine (G), and thymine (T) were synthesized on a gram scale using an iterative phosphorylation strategy that avoids the need for tedious HPLC purification. This new synthetic procedure greatly increases the scale on which tNTP substrates can be produced for polymerase-mediated TNA synthesis studies.