| Literature DB >> 28759728 |
Chen Zhu1,1, Yanzhen Wang1,1, Chunhuan Chen1,1, Changyou Wang1,1, Aicen Zhang1,1, Nana Peng1,1, Yajuan Wang1,1, Hong Zhang1,1, Xinlun Liu1,1, Wanquan Ji1,1.
Abstract
Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.Entities:
Keywords: FISH; GISH; Thinopyrum ponticum; Triticum aestivum; marqueurs moléculaires; molecular markers; résistance à la rouille striée; stripe rust resistance
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Year: 2017 PMID： 28759728 DOI： 10.1139/gen-2017-0099
Source DB: PubMed Journal: Genome ISSN： 0831-2796 Impact factor: 2.166