Suping Wang1, Haohao Wang1, Biao Ren2, Hao Li2, Michael D Weir3, Xuedong Zhou2, Thomas W Oates3, Lei Cheng4, Hockin H K Xu5. 1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA. 2. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. 3. Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA. 4. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA. Electronic address: chenglei@scu.edu.cn. 5. Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore County, MD 21250, USA. Electronic address: hxu@umaryland.edu.
Abstract
OBJECTIVES: Antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (QAMs) would induce drug resistance in oral bacteria. The objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (DMAHDM) and dimethylaminododecyl methacrylate (DMADDM) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first time. METHODS: Streptococcus mutans (S. mutans), Streptococcus sanguis, Streptococcus gordonii, Enterococcus faecalis (E. faecalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Fusobacterium nucleatum (F. nucleatum), Porphyromonas gingivalis (P. gingivalis), and Prevotella intermedia (P. intermedia) were tested. Minimum inhibitory concentration (MIC) was assessed using chlorhexidine (CHX) as control. Minimal bactericidal concentration (MBC), bacterial growth and membrane permeability properties were also investigated. RESULTS: CHX induced drug resistance in four species. DMAHDM did not induce any resistance. DMADDM induced drug resistance in only one benign species S. gordonii. The DMADDM-resistant and CHX-resistant S. gordonii had the same MIC and MBC values as S. gordonii parental strain against DMAHDM (p>0.1), hence DMAHDM effectively inhibited the resistant strains. The resistant strains had slower growth metabolism than parental strain. SIGNIFICANCE: DMAHDM induced no drug resistance, and DMADDM had much less drug resistance than the commonly-used CHX in the eight common oral species. With its potent antimicrobial functions shown previously, the new DMAHDM is promising for applications in restorative, preventive, periodontal and endodontic treatments to combat cariogenic and pathological bacteria with no drug resistance in all tested species.
OBJECTIVES: Antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (QAMs) would induce drug resistance in oral bacteria. The objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (DMAHDM) and dimethylaminododecyl methacrylate (DMADDM) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first time. METHODS:Streptococcus mutans (S. mutans), Streptococcus sanguis, Streptococcus gordonii, Enterococcus faecalis (E. faecalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Fusobacterium nucleatum (F. nucleatum), Porphyromonas gingivalis (P. gingivalis), and Prevotella intermedia (P. intermedia) were tested. Minimum inhibitory concentration (MIC) was assessed using chlorhexidine (CHX) as control. Minimal bactericidal concentration (MBC), bacterial growth and membrane permeability properties were also investigated. RESULTS:CHX induced drug resistance in four species. DMAHDM did not induce any resistance. DMADDM induced drug resistance in only one benign species S. gordonii. The DMADDM-resistant and CHX-resistant S. gordonii had the same MIC and MBC values as S. gordonii parental strain against DMAHDM (p>0.1), hence DMAHDM effectively inhibited the resistant strains. The resistant strains had slower growth metabolism than parental strain. SIGNIFICANCE: DMAHDM induced no drug resistance, and DMADDM had much less drug resistance than the commonly-used CHX in the eight common oral species. With its potent antimicrobial functions shown previously, the new DMAHDM is promising for applications in restorative, preventive, periodontal and endodontic treatments to combat cariogenic and pathological bacteria with no drug resistance in all tested species.
Authors: Sophia R Schwarz; Stefanie Hirsch; Andreas Hiergeist; Christian Kirschneck; Denise Muehler; Karl-Anton Hiller; Tim Maisch; Ali Al-Ahmad; André Gessner; Wolfgang Buchalla; Fabian Cieplik Journal: Clin Oral Investig Date: 2020-10-08 Impact factor: 3.573
Authors: Xiaoyu Huang; Yang Ge; Bina Yang; Qi Han; Wen Zhou; Jingou Liang; Mingyun Li; Xian Peng; Biao Ren; Bangcheng Yang; Michael D Weir; Qiang Guo; Haohao Wang; Xinxuan Zhou; Xugang Lu; Thomas W Oates; Hockin H K Xu; Dongmei Deng; Xuedong Zhou; Lei Cheng Journal: Bioact Mater Date: 2021-05-15
Authors: Suping Wang; Haohao Wang; Biao Ren; Xiaodong Li; Lin Wang; Han Zhou; Michael D Weir; Xuedong Zhou; Radi M Masri; Thomas W Oates; Lei Cheng; Hockin H K Xu Journal: Sci Rep Date: 2018-04-03 Impact factor: 4.379
Authors: Ke Zhang; Suping Wang; Chenchen Zhou; Lei Cheng; Xianling Gao; Xianju Xie; Jirun Sun; Haohao Wang; Michael D Weir; Mark A Reynolds; Ning Zhang; Yuxing Bai; Hockin H K Xu Journal: Bone Res Date: 2018-10-22 Impact factor: 13.567