Literature DB >> 28752454

Periodic Acid-Schiff Staining with Diastase.

Dongtao A Fu1, Martha Campbell-Thompson2.   

Abstract

Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.

Entities:  

Keywords:  Alpha-1 antitrypsin deficiency; Human liver; Periodic Acid-Schiff; Periodic Acid-Schiff reaction-diastase; Special stain

Mesh：

Substances：

Year:  2017        PMID： 28752454     DOI： 10.1007/978-1-4939-7163-3_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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