Literature DB >> 2875078

Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.

N M Kumar, N B Gilula.   

Abstract

An extended synthetic oligonucleotide (58-mer) has been used to identify and characterize a human liver gap junction cDNA. The cDNA is 1,574 bases long and contains the entire coding region for a gap junction protein. In vitro translation of the RNA products of this cDNA is consistent with it coding for a 32,022-D protein. Southern blot analysis indicates that the gap junction gene is present as a single copy, and that it can be detected in a variety of organisms using the human liver cDNA as a probe. The human cDNA has been used to screen a rat liver cDNA library, and a rat liver junction cDNA clone has been isolated. The rat liver clone is 1,127 bases in length, and it has strong sequence homology to the human cDNA in the protein-coding region, but less extensive homology in the 3'-untranslated region.

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Year:  1986        PMID： 2875078      PMCID： PMC2114303          DOI： 10.1083/jcb.103.3.767

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  49 in total

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4.  Extraction of nucleic acids from agarose gels.

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Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

6.  Recognition of cap structure in splicing in vitro of mRNA precursors.

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8.  Analysis of vertebrate gap junction protein.

Authors:  M E Finbow; J Shuttleworth; A E Hamilton; J D Pitts
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Authors:  E L Hertzberg; D J Anderson; M Friedlander; N B Gilula
Journal:  J Cell Biol       Date:  1982-01       Impact factor: 10.539

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Authors:  J P Revel; M J Karnovsky
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Review 6.  Molecular modeling and mutagenesis of gap junction channels.

Authors:  Julio A Kovacs; Kent A Baker; Guillermo A Altenberg; Ruben Abagyan; Mark Yeager
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10.  Connexin 32 mutations from X-linked Charcot-Marie-Tooth disease patients: functional defects and dominant negative effects.

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