Literature DB >> 2874553

Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.

E E Baetge, Y H Suh, T H Joh.   

Abstract

We report here the isolation of a cDNA clone containing the full coding region of bovine phenylethanolamine N-methyltransferase (PNMTase, EC 2.1.1.28, S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase). The complete nucleotide sequence of the cDNA has been determined, and the amino acid sequence of PNMTase deduced. Cultured cells transfected with an expression vector containing this cDNA produced high levels of PNMTase enzymatic activity. Antibodies specific for tyrosine hydroxylase [EC 1.14.16.2, tyrosine 3-monooxygenase; L-tyrosine, tetrahydrobiopterine: oxygen oxidoreductase (3-hydroxylating)], the first enzyme in the catecholamine pathway, possess a striking affinity for the PNMTase protein synthesized in vitro. Comparison of the deduced amino acid sequence of bovine PNMTase to rat tyrosine hydroxylase reveals that PNMTase shares significant homology with tyrosine hydroxylase and supports previous protein and immunological data suggesting that the catecholamine biosynthetic enzymes are structurally related.

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Year:  1986        PMID： 2874553      PMCID： PMC386305          DOI： 10.1073/pnas.83.15.5454

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  39 in total

1.  Purification and properties of phenylethanolamine-N-methyl transferase.

Authors:  J AXELROD
Journal:  J Biol Chem       Date:  1962-05       Impact factor: 5.157

2.  Monoamine-synthesizing enzymes in central dopaminergic, noradrenergic and serotonergic neurons. Immunocytochemical localization by light and electron microscopy.

Authors:  V M Pickel; T H Joh; D J Reis
Journal:  J Histochem Cytochem       Date:  1976-07       Impact factor: 2.479

3.  Localisation of phenylethanolamine N-methyl transferase in the rat brain nuclei.

Authors:  J M Saavedra; M Palkovits; M J Brownstein; J Axelrod
Journal:  Nature       Date:  1974-04-19       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Internal homologies in the two aspartokinase-homoserine dehydrogenases of Escherichia coli K-12.

Authors:  P Ferrara; N Duchange; M M Zakin; G N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

6.  Immunohistochemical studies on phenylethanolamine-N-methyltransferase, dopa-decarboxylase and dopamine- -hydroxylase.

Authors:  M Goldstein; K Fuxe; T Hökfelt; T H Joh
Journal:  Experientia       Date:  1971-08

7.  Subcellular site of biosynthesis of the catecholamine biosynthetic enzymes in bovine adrenal medulla.

Authors:  E L Sabban; M Goldstein
Journal:  J Neurochem       Date:  1984-12       Impact factor: 5.372

8.  Regulation, linkage, and sequence of mouse metallothionein I and II genes.

Authors:  P F Searle; B L Davison; G W Stuart; T M Wilkie; G Norstedt; R D Palmiter
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

9.  A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene.

Authors:  G W Stuart; P F Searle; H Y Chen; R L Brinster; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

10.  Cholinergic neurons of the chick ciliary ganglia express adrenergic traits in vivo and in vitro.

Authors:  G Teitelman; T H Joh; L Grayson; D H Park; D J Reis; L Iacovitti
Journal:  J Neurosci       Date:  1985-01       Impact factor: 6.167
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  15 in total

1.  Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines.

Authors:  T Tanaka; Y Horio; M Taketoshi; I Imamura; M Ando-Yamamoto; K Kangawa; H Matsuo; M Kuroda; H Wada
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

2.  Coexistence and gene expression of phenylethanolamine N-methyltransferase, tyrosine hydroxylase, and neuropeptide tyrosine in the rat and bovine adrenal gland: effects of reserpine.

Authors:  M Schalling; A Dagerlind; S Brené; H Hallman; M Djurfeldt; H Persson; L Terenius; M Goldstein; D Schlesinger; T Hökfelt
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

3.  Differential and coordinate regulation of TH and PNMT mRNAs in chromaffin cell cultures by second messenger system activation and steroid treatment.

Authors:  J M Carroll; M J Evinger; H M Goodman; T H Joh
Journal:  J Mol Neurosci       Date:  1991       Impact factor: 3.444

4.  Full-length cDNA for rabbit tryptophan hydroxylase: functional domains and evolution of aromatic amino acid hydroxylases.

Authors:  H E Grenett; F D Ledley; L L Reed; S L Woo
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

5.  Cloning of the rat adrenal medullary phenylethanolamine-N-methyltransferase.

Authors:  E Mezey
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

6.  Pituitary adenylate cyclase activating polypeptide (PACAP) regulates expression of catecholamine biosynthetic enzyme genes in bovine adrenal chromaffin cells.

Authors:  C Tönshoff; L Hemmick; M J Evinger
Journal:  J Mol Neurosci       Date:  1997-10       Impact factor: 3.444

7.  GDNF reduces drug-induced rotational behavior after medial forebrain bundle transection by a mechanism not involving striatal dopamine.

Authors:  J L Tseng; E E Baetge; A D Zurn; P Aebischer
Journal:  J Neurosci       Date:  1997-01-01       Impact factor: 6.167

8.  Effects of cAMP, glucocorticoids, and calcium on dopamine beta-hydroxylase gene expression in bovine chromaffin cells.

Authors:  O Hwang; T H Joh
Journal:  J Mol Neurosci       Date:  1993       Impact factor: 3.444

9.  Glucocorticoids stimulate transcription of the rat phenylethanolamine N-methyltransferase (PNMT) gene in vivo and in vitro.

Authors:  M J Evinger; A C Towle; D H Park; P Lee; T H Joh
Journal:  Cell Mol Neurobiol       Date:  1992-06       Impact factor: 5.046

10.  Molecular cloning, sequence analysis, and expression in Escherichia coli of the cDNA for guanidinoacetate methyltransferase from rat liver.

Authors:  H Ogawa; T Date; T Gomi; K Konishi; H C Pitot; G L Cantoni; M Fujioka
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205
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