Fengchao Li1, Yingqian Zhang1, Jing Bai1, Marc M Greenberg2, Zhen Xi1,3, Chuanzheng Zhou1,3. 1. State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, College of Chemistry, Nankai University , Tianjin 300071, China. 2. Department of Chemistry, Johns Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States. 3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
Abstract
In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.
In situ generation of 5-formylcytosine (n class="Chemical">5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.
Authors: Shaofei Ji; Hongzhao Shao; Qiyuan Han; Christopher L Seiler; Natalia Y Tretyakova Journal: Angew Chem Int Ed Engl Date: 2017-10-06 Impact factor: 15.336