Jianwei He1, Weiwei Cao2, Inayat Azeem3, Qiang Zhao4, Zengwu Shao5. 1. First Affiliated Hospital of Shihezi University, School of Medicine, Xinjiang, China; Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: hjw5566@126.com. 2. Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, School of Medicine, Xinjiang, China. 3. Office for Education to International Students, School of Medicine, Shihezi University, Xinjiang, China. 4. Department of Cardiovascular Internal Medicine, First Affiliated Hospital of Xinjiang University, School of Medicine, Xinjiang, China. 5. Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: szwjj@medmail.com.cn.
Abstract
BACKGROUND: Associations between Transforming Growth Factor beta-1 (TGF-β1), Platelet-derived growth factor BB (PDGF-BB) and C-terminal telopeptide of type I collagen (CTX-1) with pathogenesis of osteoarthritis (OA) have already been established. Therefore, TGF-β1, PDGF-BB and CTX-1 could be used as the potential biomarkers for the diagnosis of this disease. METHODS: 160 knee OA cases and 80 healthy controls were chosen. Serum concentrations of TGF-β1, PDGF-BB and CTX-1 were tested twice on the same samples using Enzyme-Linked Immunosorbent Assay. Knee OA cases were classified using Kellgren-Lawrence (K-L) criteria into four groups. Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index scores were evaluated for all cases. RESULTS: TGF-β1 serum levels in knee OA cases were significantly higher compared to controls (t=11.37, p<0.01). Average serum level of TGF-β1 was positively associated with K-L grades. The diagnostic efficiency of serum TGF-β1 was proved to be moderate and the cut-point value was 12.11ng/ml showing favorable diagnostic indexes. In contrast, similarities were found not only in serum levels of PDGF-BB and CTX-1 between OA groups and controls but also in WOMAC scores. CONCLUSION: Our findings supported that TGF-β1 has a positive correlation with the severity of knee OA confirmed by radiographs. Therefore, TGF-β1 might serve as a potential biomarker in established knee OA.
BACKGROUND: Associations between Transforming Growth Factor beta-1 (TGF-β1), Platelet-derived growth factor BB (PDGF-BB) and C-terminal telopeptide of type I collagen (CTX-1) with pathogenesis of osteoarthritis (OA) have already been established. Therefore, TGF-β1, PDGF-BB and CTX-1 could be used as the potential biomarkers for the diagnosis of this disease. METHODS: 160 knee OA cases and 80 healthy controls were chosen. Serum concentrations of TGF-β1, PDGF-BB and CTX-1 were tested twice on the same samples using Enzyme-Linked Immunosorbent Assay. Knee OA cases were classified using Kellgren-Lawrence (K-L) criteria into four groups. Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index scores were evaluated for all cases. RESULTS: TGF-β1 serum levels in knee OA cases were significantly higher compared to controls (t=11.37, p<0.01). Average serum level of TGF-β1 was positively associated with K-L grades. The diagnostic efficiency of serum TGF-β1 was proved to be moderate and the cut-point value was 12.11ng/ml showing favorable diagnostic indexes. In contrast, similarities were found not only in serum levels of PDGF-BB and CTX-1 between OA groups and controls but also in WOMAC scores. CONCLUSION: Our findings supported that TGF-β1 has a positive correlation with the severity of knee OA confirmed by radiographs. Therefore, TGF-β1 might serve as a potential biomarker in established knee OA.