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Literature DB >> 2873789

Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

Abstract

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.

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Year: 1986 PMID： 2873789 PMCID： PMC203446 DOI： 10.1128/aem.52.1.197-199.1986

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

8 in total

1. Isolation of covalently closed circular DNA of high molecular weight from bacteria.

Authors: T C Currier; E W Nester
Journal: Anal Biochem Date: 1976-12 Impact factor: 3.365

2. Isolation and characterization of multiply antibiotic-resistant Clostridum perfringens strains from porcine feces.

Authors: J I Rood; E A Maher; E B Somers; E Campos; C L Duncan
Journal: Antimicrob Agents Chemother Date: 1978-05 Impact factor: 5.191

3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

4. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

5. Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B.

Authors: H P Blaschek; M Solberg
Journal: J Bacteriol Date: 1981-07 Impact factor: 3.490

6. Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Authors: D G Anderson; L L McKay
Journal: Appl Environ Microbiol Date: 1983-09 Impact factor: 4.792

7. Role of DNase in recovery of plasmid DNA from Clostridium perfringens.

Authors: H P Blaschek; M A Klacik
Journal: Appl Environ Microbiol Date: 1984-07 Impact factor: 4.792

8. Shuttle plasmids for Escherichia coli and Clostridium perfringens.

Authors: C H Squires; D L Heefner; R J Evans; B J Kopp; M J Yarus
Journal: J Bacteriol Date: 1984-08 Impact factor: 3.490

8 in total

16 in total

1. Screening for plasmids in the genus Clostridium.

Authors: C K Lee; P Dürre; H Hippe; G Gottschalk
Journal: Arch Microbiol Date: 1987-07 Impact factor: 2.552

2. A comparison of methods for isolating plasmid DNA from Clostridium perfringens.

Authors: A Hussain
Journal: Vet Res Commun Date: 1993 Impact factor: 2.459

3. Molecular cloning and genetic analysis of a chloramphenicol acetyltransferase determinant from Clostridium difficile.

Authors: B W Wren; P Mullany; C Clayton; S Tabaqchali
Journal: Antimicrob Agents Chemother Date: 1988-08 Impact factor: 5.191

4. A Molecular Biology Tool Kit for the Phototrophic Firmicute Heliobacterium modesticaldum.

Authors: Patricia L Baker; Gregory S Orf; Zahid Khan; Levi Espinoza; Sabrina Leung; Kimberly Kevershan; Kevin E Redding
Journal: Appl Environ Microbiol Date: 2019-09-17 Impact factor: 4.792

Review 5. Molecular genetics and pathogenesis of Clostridium perfringens.

Authors: J I Rood; S T Cole
Journal: Microbiol Rev Date: 1991-12

6. Role of alpha-toxin in Clostridium perfringens infection determined by using recombinants of C. perfringens and Bacillus subtilis.

Authors: M Ninomiya; O Matsushita; J Minami; H Sakamoto; M Nakano; A Okabe
Journal: Infect Immun Date: 1994-11 Impact factor: 3.441

7. Purification, characterization, and primary structure of Clostridium perfringens lambda-toxin, a thermolysin-like metalloprotease.

Authors: F Jin; O Matsushita; S Katayama; S Jin; C Matsushita; J Minami; A Okabe
Journal: Infect Immun Date: 1996-01 Impact factor: 3.441