| Literature DB >> 28735115 |
Xiaoyan Cui1, Hoda Yaghmaiean2, Guanwei Wu1, Xiaoyun Wu3, Xin Chen4, Greg Thorn5, Aiming Wang6.
Abstract
Like other positive-strand RNA viruses, plant potyviruses assemble viral replication complexes (VRCs) on modified cellular membranes. Potyviruses encode two membrane proteins, 6K2 and P3. The former is known to play pivotal roles in the formation of membrane-associated VRCs. However, P3 remains to be one of the least characterized potyviral proteins. The P3 cistron codes for P3 as well as P3N-PIPO which results from RNA polymerase slippage. In this study, we show that the P3N-PIPO of Turnip mosaic virus (TuMV) is required for viral cell-to-cell movement but not for viral replication. We demonstrate that the C-terminal region of P3 (P3C) is indispensable for P3 to form cytoplasmic punctate inclusions and target VRCs. We reveal that TuMV mutants that lack P3C are replication-defective. Taken together, these data suggest that the P3 cistron has two distinct functions: P3N-PIPO as a dedicated movement protein and P3 as an essential component of the VRC. CrownEntities:
Keywords: Cell-to-cell movement; Movement protein; P3; P3N-PIPO; Plant RNA virus; Potyvirus; Punctate inclusion; Turnip mosaic virus; Virus replication complex
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Year: 2017 PMID： 28735115 DOI： 10.1016/j.virol.2017.07.016
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616