Xiaojuan Wang1,2, Yisong Chen1,2, Zhongyong Fan3, Keqin Hua4,5. 1. Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 419 Fang-Xie Road, Shanghai, 200011, People's Republic of China. 2. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 413 Zhao-Zhou Road, Shanghai, 200011, People's Republic of China. 3. Department of Materials Science, Fudan University, Handan Road No. 220, Shanghai, 200433, People's Republic of China. 4. Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 419 Fang-Xie Road, Shanghai, 200011, People's Republic of China. huakeqinjiaoshou@163.com. 5. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 413 Zhao-Zhou Road, Shanghai, 200011, People's Republic of China. huakeqinjiaoshou@163.com.
Abstract
INTRODUCTION AND HYPOTHESIS: Synthetic non-absorbable meshes are widely used to augment surgical repair of pelvic organ prolapse (POP) and stress urinary incontinence (SUI), but these meshes are associated with serious complications. This study compares the attachment and extracellular matrix (ECM) production of adipose-derived stem cells (ADSCs) on different biodegradable nanomaterials to develop tissue engineered repair materials. METHODS: Rat ADSCs were isolated and cultured on electrospun poly-L-lactic acid (PLA) and electrospun poly(L-lactide)-trimethylene carbonate-gycolide (PLTG) terpolymers for 1 and 2 weeks. Samples were tested for cell proliferation (cell counting kit-8), microstructure, and morphology (scanning electron microscopy), production of ECM components (immunostaining for collagen I, collagen III, and elastin) and biomechanical properties (uniaxial tensile methods). RESULTS: The ADSCs showed good attachment and proliferation on both PLA and PLTG scaffolds. The production of collagen I and collagen III on both scaffolds was greater at 14 days than at 7 days and was greater on PLTG scaffolds than on PLA scaffolds, but these differences were not significant. The addition of ADSCs onto scaffolds led to a significant increase in the biomechanical properties of both PLA and PLTG scaffolds compared with unseeded scaffolds. CONCLUSION: These data support the use of both PLA and PLTG as tissue-engineered repair materials for POP or SUI.
INTRODUCTION AND HYPOTHESIS: Synthetic non-absorbable meshes are widely used to augment surgical repair of pelvic organ prolapse (POP) and stress urinary incontinence (SUI), but these meshes are associated with serious complications. This study compares the attachment and extracellular matrix (ECM) production of adipose-derived stem cells (ADSCs) on different biodegradable nanomaterials to develop tissue engineered repair materials. METHODS:Rat ADSCs were isolated and cultured on electrospun poly-L-lactic acid (PLA) and electrospun poly(L-lactide)-trimethylene carbonate-gycolide (PLTG) terpolymers for 1 and 2 weeks. Samples were tested for cell proliferation (cell counting kit-8), microstructure, and morphology (scanning electron microscopy), production of ECM components (immunostaining for collagen I, collagen III, and elastin) and biomechanical properties (uniaxial tensile methods). RESULTS: The ADSCs showed good attachment and proliferation on both PLA and PLTG scaffolds. The production of collagen I and collagen III on both scaffolds was greater at 14 days than at 7 days and was greater on PLTG scaffolds than on PLA scaffolds, but these differences were not significant. The addition of ADSCs onto scaffolds led to a significant increase in the biomechanical properties of both PLA and PLTG scaffolds compared with unseeded scaffolds. CONCLUSION: These data support the use of both PLA and PLTG as tissue-engineered repair materials for POP or SUI.
Entities:
Keywords:
Pelvic organ prolapse; Poly(L-lactide)-trimethylene carbonate-gycolide; Poly-L-lactic acid; Stress urinary incontinence; Tissue engineered repair material
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