Anders Henningsen1,2, Ralf Smeets3, Philip Hartjen3, Oliver Heinrich3, Roman Heuberger4, Max Heiland5, Clarissa Precht3, Claudio Cacaci6. 1. Department of Oral and Maxillofacial Surgery, University Hospital Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany. a.henningsen@uke.de. 2. Department of Oral and Maxillofacial Surgery, German Armed Forces Hospital, Lesserstrasse 180, 22049, Hamburg, Germany. a.henningsen@uke.de. 3. Department of Oral and Maxillofacial Surgery, University Hospital Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany. 4. RMS Foundation, Bischmattstraße 12, 2544, Bettlach, Switzerland. 5. Department of Oral and Maxillofacial Surgery, Charité University Hospital, Augustenburger Platz 1, 13353, Berlin, Germany. 6. Implant Competence Centrum, Weinstr. 4, 80333, Munich, Germany.
Abstract
OBJECTIVE: The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization. MATERIALS AND METHODS: Moderately rough (Ra 1.8-2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm2 at λ = 360 nm and 2 mW/cm2 at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each. Surface structure was investigated by scanning electron microscopy, confocal microscopy and X-ray photoelectron spectroscopy (XPS). Hydrophilicity was assessed by dynamic contact angle measurement. Cell attachment, viability, cell proliferation and cytotoxicity were assessed in vitro using murine osteoblast-like cells. RESULTS: UV irradiation or NTP treatment of titanium surfaces did not alter the surface structure. XPS analysis revealed a significantly increased oxidation of the surface and a decrease of carbon after the use of either method. NTP and UV light led to a significant better cell attachment of murine osteoblasts; significantly more osteoblasts grew on the treated surfaces at each time point (p < 0.001). CONCLUSIONS: UV light as well as NTP modified the surface of titanium and significantly improved the conditions for murine osteoblast cells in vitro. However, results indicate a slight advantage for NTP of argon and oxygen in a short time interval of surface functionalization compared to UV. CLINICAL RELEVANCE: UV light and NTP are able to improve surface conditions of dental implants made of titanium.
OBJECTIVE: The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization. MATERIALS AND METHODS: Moderately rough (Ra 1.8-2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm2 at λ = 360 nm and 2 mW/cm2 at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each. Surface structure was investigated by scanning electron microscopy, confocal microscopy and X-ray photoelectron spectroscopy (XPS). Hydrophilicity was assessed by dynamic contact angle measurement. Cell attachment, viability, cell proliferation and cytotoxicity were assessed in vitro using murine osteoblast-like cells. RESULTS: UV irradiation or NTP treatment of titanium surfaces did not alter the surface structure. XPS analysis revealed a significantly increased oxidation of the surface and a decrease of carbon after the use of either method. NTP and UV light led to a significant better cell attachment of murine osteoblasts; significantly more osteoblasts grew on the treated surfaces at each time point (p < 0.001). CONCLUSIONS: UV light as well as NTP modified the surface of titanium and significantly improved the conditions for murine osteoblast cells in vitro. However, results indicate a slight advantage for NTP of argon and oxygen in a short time interval of surface functionalization compared to UV. CLINICAL RELEVANCE: UV light and NTP are able to improve surface conditions of dental implants made of titanium.
Authors: Paulo G Coelho; Gabriela Giro; Hellen S Teixeira; Charles Marin; Lukas Witek; Van P Thompson; Nick Tovar; Nelson R F A Silva Journal: J Biomed Mater Res A Date: 2012-04-10 Impact factor: 4.396
Authors: Eduardo Mariscal-Muñoz; Carlos A S Costa; Hewerson S Tavares; Jonas Bianchi; Josimeri Hebling; João P B Machado; Ulf H Lerner; Pedro P C Souza Journal: Clin Oral Investig Date: 2015-07-30 Impact factor: 3.573
Authors: Linna Guo; Ralf Smeets; Lan Kluwe; Philip Hartjen; Mike Barbeck; Claudio Cacaci; Martin Gosau; Anders Henningsen Journal: Int J Mol Sci Date: 2019-11-08 Impact factor: 5.923
Authors: Linna Guo; Ziang Zou; Ralf Smeets; Lan Kluwe; Philip Hartjen; Claudio Cacaci; Martin Gosau; Anders Henningsen Journal: Int J Mol Sci Date: 2020-11-14 Impact factor: 5.923