Yang Yang1, Miao Zheng2, Yang Yang1, Jing Li3, Yong-Fei Su4, He-Ping Li5, Jian-Guo Tan6. 1. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, People's Republic of China. 2. Department of Stomatology, Peking University Third Hospital, Beijing, 100191, People's Republic of China. 3. Department of Engineering Physics, Tsinghua University, Beijing, 100084, People's Republic of China. 4. College of Mechanical Engineering, North China University of Science and Technology, Tangshan, 063210, People's Republic of China. 5. Department of Engineering Physics, Tsinghua University, Beijing, 100084, People's Republic of China. liheping@tsinghua.edu.cn. 6. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, People's Republic of China. kqtanjg@bjmu.edu.cn.
Abstract
OBJECTIVES: This study presents a surface modification method to treat the zirconia implant abutment materials using a helium cold atmospheric plasma (CAP) jet in order to evaluate its efficacy on oral bacteria adhesion and growth. MATERIALS AND METHODS: Yttrium-Stabilized Zirconia disks were subjected to helium CAP treatment; after the treatment, zirconia surface was evaluated using scanning electron microscopy, a contact angle measuring device, X-ray photoelectron spectroscopy for surface characteristics. The response of Streptococcus mutans and Porphyromonas gingivalis on treated surface was evaluated by a scanning electron microscopy, MTT assay, and LIVE/DEAD staining. The biofilm formation was analyzed using a crystal violet assay. RESULTS: After the helium CAP jet treatment, the zirconia surface chemistry has been changed while the surface topography remains unchanged, the bacterial growth was inhibited, and the biofilm forming decreased. As the treatment time increases, the zirconia abutment showed a better bacterial inhibition efficacy. CONCLUSIONS: The helium CAP jet surface modification approach can eliminate bacterial growth on zirconia surface with surface chemistry change, while surface topography remained. CLINICAL RELEVANCE: Soft tissue seal around dental implant abutment plays a crucial role in maintaining long-term success. However, it is weaker than periodontal barriers and vulnerable to bacterial invasion. CAP has a potential prospect for improving soft tissue seal around the zirconia abutment, therefore providing better esthetics and most of all, prevent peri-implant lesions from happening.
OBJECTIVES: This study presents a surface modification method to treat the zirconia implant abutment materials using a helium cold atmospheric plasma (CAP) jet in order to evaluate its efficacy on oral bacteria adhesion and growth. MATERIALS AND METHODS: Yttrium-Stabilized Zirconia disks were subjected to helium CAP treatment; after the treatment, zirconia surface was evaluated using scanning electron microscopy, a contact angle measuring device, X-ray photoelectron spectroscopy for surface characteristics. The response of Streptococcus mutans and Porphyromonas gingivalis on treated surface was evaluated by a scanning electron microscopy, MTT assay, and LIVE/DEAD staining. The biofilm formation was analyzed using a crystal violet assay. RESULTS: After the helium CAP jet treatment, the zirconia surface chemistry has been changed while the surface topography remains unchanged, the bacterial growth was inhibited, and the biofilm forming decreased. As the treatment time increases, the zirconia abutment showed a better bacterial inhibition efficacy. CONCLUSIONS: The helium CAP jet surface modification approach can eliminate bacterial growth on zirconia surface with surface chemistry change, while surface topography remained. CLINICAL RELEVANCE: Soft tissue seal around dental implant abutment plays a crucial role in maintaining long-term success. However, it is weaker than periodontal barriers and vulnerable to bacterial invasion. CAP has a potential prospect for improving soft tissue seal around the zirconia abutment, therefore providing better esthetics and most of all, prevent peri-implant lesions from happening.
Authors: Tullio Genova; Paolo Pesce; Federico Mussano; Kazushige Tanaka; Luigi Canullo Journal: J Biomed Mater Res A Date: 2018-10-25 Impact factor: 4.396
Authors: Berta Garcia; Fabio Camacho; David Peñarrocha; Marco Tallarico; Sara Perez; Luigi Canullo Journal: Clin Oral Implants Res Date: 2016-08-23 Impact factor: 5.977
Authors: Ana Stefany Meza-Siccha; Miguel Angel Aguilar-Luis; Wilmer Silva-Caso; Fernando Mazulis; Carolina Barragan-Salazar; Juana Del Valle-Mendoza Journal: Int J Dent Date: 2019-06-13