| Literature DB >> 28730434 |
Maria L Kireeva1, Kirill A Afonin2,3,4, Bruce A Shapiro2,5, Mikhail Kashlev2.
Abstract
RNA nanoparticles consisting of multiple RNA strands of different sequences forming various three-dimensional structures emerge as promising carriers of siRNAs, RNA aptamers, and ribozymes. In vitro transcription of a mixture of dsDNA templates encoding all the subunits of the RNA nanoparticle may result in cotranscriptional self-assembly of the nanoparticle. Based on our experience with production of RNA nanorings, RNA nanocubes, and RNA three-way junctions, we propose a strategy for optimization of the cotranscriptional production of chemically modified ribonuclease-resistant RNA nanoparticles.Entities:
Keywords: 2′-F-dCTP; 2′-F-dUTP; Cotranscriptional assembly; In vitro transcription; RNA nanocubes; RNA nanorings; T7 R&DNA polymerase; T7 RNA polymerase
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Year: 2017 PMID： 28730434 PMCID： PMC7927353 DOI： 10.1007/978-1-4939-7138-1_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745