Literature DB >> 2871626

Plant glutamine synthetase complements a glnA mutation in Escherichia coli.

S DasSarma, E Tischer, H M Goodman.   

Abstract

A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.

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Year:  1986        PMID： 2871626     DOI： 10.1126/science.2871626

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  20 in total

1.  Constitutive overexpression of cytosolic glutamine synthetase (GS1) gene in transgenic alfalfa demonstrates that GS1 may be regulated at the level of RNA stability and protein turnover.

Authors:  J L Ortega; S J Temple; C Sengupta-Gopalan
Journal:  Plant Physiol       Date:  2001-05       Impact factor: 8.340

2.  Biochemical and molecular characterization of transgenic Lotus japonicus plants constitutively over-expressing a cytosolic glutamine synthetase gene.

Authors:  Jose Luis Ortega; Stephen J Temple; Suman Bagga; Soumitra Ghoshroy; Champa Sengupta-Gopalan
Journal:  Planta       Date:  2004-06-10       Impact factor: 4.116

3.  Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli.

Authors:  S K Lal; S Johnson; T Conway; P M Kelley
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076

4.  Down-regulation of specific members of the glutamine synthetase gene family in alfalfa by antisense RNA technology.

Authors:  S J Temple; S Bagga; C Sengupta-Gopalan
Journal:  Plant Mol Biol       Date:  1998-06       Impact factor: 4.076

5.  Genetic determinants of glutamine synthetase in Drosophila melanogaster: a gene for glutamine synthetase I resides in the 21B3-6 region.

Authors:  C Caggese; R Caizzi; M P Bozzetti; P Barsanti; F Ritossa
Journal:  Biochem Genet       Date:  1988-10       Impact factor: 1.890

6.  Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli.

Authors:  D P Snustad; J P Hunsperger; B M Chereskin; J Messing
Journal:  Genetics       Date:  1988-12       Impact factor: 4.562

7.  Heteromeric assembly of the cytosolic glutamine synthetase polypeptides of Medicago truncatula: complementation of a glnA Escherichia coli mutant with a plant domain-swapped enzyme.

Authors:  H Carvalho; C Sunkel; R Salema; J V Cullimore
Journal:  Plant Mol Biol       Date:  1997-11       Impact factor: 4.076

8.  A soybean gene encoding delta 1-pyrroline-5-carboxylate reductase was isolated by functional complementation in Escherichia coli and is found to be osmoregulated.

Authors:  A J Delauney; D P Verma
Journal:  Mol Gen Genet       Date:  1990-05

9.  Several proteins imported into chloroplasts form stable complexes with the GroEL-related chloroplast molecular chaperone.

Authors:  T H Lubben; G K Donaldson; P V Viitanen; A A Gatenby
Journal:  Plant Cell       Date:  1989-12       Impact factor: 11.277

10.  Complementation of a polyamine-deficient Escherichia coli mutant by expression of mouse ornithine decarboxylase.

Authors:  M Macrae; P Coffino
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272
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