Vera Lukasova1,2, Matej Buzgo3,4, Vera Sovkova2,3, Jana Dankova2,3, Michala Rampichova2,4, Evzen Amler2,3,4. 1. Faculty of Science, Charles University in Prague, Prague, Czech Republic. 2. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. 3. Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. 4. University Center for Energy Efficient Buildings, Czech Technical University in Prague, Bustehrad, Czech Republic.
Abstract
OBJECTIVES: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs). MATERIALS AND METHODS: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement. Analysis of pMSCs cultures included measurement of the metabolic activity and proliferation, immunofluorescence staining and also qPCR. RESULTS: Results showed no detrimental effect of the bioactive peptides to cultured pMSCs. Based on qPCR analysis, the bioactive peptides are specific for osteogenic differentiation with no detectable expression of collagen II. Our results further indicate that peptide derived from BMP-2 protein promoted the expression of mRNA for osteocalcin (OCN) and collagen I significantly compared to control groups and also supported deposition of OCN as observed by immunostaining method. CONCLUSION: The data suggest that bioactive peptide with an amino acid sequence of KIPKASSVPTELSAISTLYL derived from BMP-2 protein was the most potent for triggering osteogenic differentiation of pMSCs.
OBJECTIVES: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs). MATERIALS AND METHODS: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement. Analysis of pMSCs cultures included measurement of the metabolic activity and proliferation, immunofluorescence staining and also qPCR. RESULTS: Results showed no detrimental effect of the bioactive peptides to cultured pMSCs. Based on qPCR analysis, the bioactive peptides are specific for osteogenic differentiation with no detectable expression of collagen II. Our results further indicate that peptide derived from BMP-2 protein promoted the expression of mRNA for osteocalcin (OCN) and collagen I significantly compared to control groups and also supported deposition of OCN as observed by immunostaining method. CONCLUSION: The data suggest that bioactive peptide with an amino acid sequence of KIPKASSVPTELSAISTLYL derived from BMP-2 protein was the most potent for triggering osteogenic differentiation of pMSCs.
Authors: Eva Balint; David Lapointe; Hicham Drissi; Caroline van der Meijden; Daniel W Young; Andre J van Wijnen; Janet L Stein; Gary S Stein; Jane B Lian Journal: J Cell Biochem Date: 2003-05-15 Impact factor: 4.429
Authors: Vineet Agrawal; Stephen Tottey; Scott A Johnson; John M Freund; Bernard F Siu; Stephen F Badylak Journal: Tissue Eng Part A Date: 2011-06-16 Impact factor: 3.845
Authors: M Rampichová; E Koštáková; E Filová; E Prosecká; M Plencner; L Ocheretná; A Lytvynets; D Lukáš; E Amler Journal: Physiol Res Date: 2010-04-20 Impact factor: 1.881
Authors: Eva Filová; Michala Rampichová; Andrej Litvinec; Milan Držík; Andrea Míčková; Matej Buzgo; Eva Košťáková; Lenka Martinová; Dušan Usvald; Eva Prosecká; Jiří Uhlík; Jan Motlík; Luděk Vajner; Evžen Amler Journal: Int J Pharm Date: 2013-03-07 Impact factor: 5.875
Authors: Bojiang Shen; Aiqun Wei; Shane Whittaker; Lisa A Williams; Helen Tao; David D F Ma; Ashish D Diwan Journal: J Cell Biochem Date: 2010-02-01 Impact factor: 4.429
Authors: Kristin M Hennessy; Beth E Pollot; William C Clem; Matthew C Phipps; Amber A Sawyer; Bonnie K Culpepper; Susan L Bellis Journal: Biomaterials Date: 2009-01-20 Impact factor: 12.479
Authors: Vera Lukasova; Matej Buzgo; Vera Sovkova; Jana Dankova; Michala Rampichova; Evzen Amler Journal: Cell Prolif Date: 2017-08 Impact factor: 6.831