| Literature DB >> 28710757 |
Candace L Minchew1,2, Vladimir V Didenko3.
Abstract
Blunt-ended DNase II-type breaks with 5' hydroxyls are generated in phagocytic cells of any lineage during digestion of the engulfed DNA. These breaks indicate the ongoing active phagocytic reaction. They are produced by the acid deoxyribonuclease-DNase II which is the primary endonuclease responsible for DNA degradation after its engulfment.Here, we present an express approach that detects blunt-ended 5' OH DNA breaks in fixed tissue sections. The technique is simple to perform and takes only 60 min to complete. It can be useful in studies of the clearance of dying cells in oncological, inflammatory, and autoimmune disorders.Entities:
Keywords: 5′ OH DNA probes; Clearance of cell death; Express detection of DNase II cleavage; Labeling of phagocytosis; Phagocytic digestion of DNA; Phagolysosomes
Mesh：
Substances：
Year: 2017 PMID： 28710757 PMCID： PMC5654645 DOI： 10.1007/978-1-4939-7187-9_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745