Tracy R Fitzsimmons1, Shaohua Ge2, P Mark Bartold3. 1. Adelaide Dental School, The University of Adelaide, North Terrace, Adelaide, SA, 5005, Australia. tracy.fitzsimmons@adelaide.edu.au. 2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration Department of Periodontology, School of Stomatology, Shandong University, Shandong Province, Jinan, China. 3. Adelaide Dental School, The University of Adelaide, North Terrace, Adelaide, SA, 5005, Australia.
Abstract
OBJECTIVES: The aims of this study were to compare the in vitro cytokine response of gingival fibroblasts (GF's) from healthy and inflamed human gingival tissues and to assess whether GF's from inflamed gingivae are capable of mounting a secondary inflammatory response after exposure to P. gingivalis LPS. MATERIALS AND METHODS: GF's were obtained from healthy donors and periodontitis patients and cultured in vitro. Cells were exposed to P. gingivalis LPS for 24h before measurement of MCP-1, GRO, IL-6, IL-8 and VEGF using a bead-based multiplex assay. Statistical comparisons were made between LPS-exposed GF's and unstimulated cells as well as the two patient groups by two-way ANOVA. RESULTS: GF's exposed to P. gingivalis LPS significantly increased their production of MCP-1, GRO, IL-6, IL-8 and VEGF compared to unstimulated cells. GF's isolated from inflamed tissue from periodontitis patients demonstrated consistently less cytokine production after exposure to P. gingivalis LPS, most notably for GRO and IL-6. CONCLUSIONS: The current study demonstrates that GF's play an active role in the inflammatory response in periodontal disease by producing a number of chemokines and cytokines. Furthermore, inflamed GF's may be compromised in their ability to mount an adequate secondary immune response in relation to chemokine/cytokine production. CLINICAL RELEVANCE: The compromised inflammatory cytokine response of inflamed human gingival fibroblasts to P. gingivalis LPS may impact on their ability to recruit and activate inflammatory cells while maintaining persistent inflammation, a key feature of periodontal disease.
OBJECTIVES: The aims of this study were to compare the in vitro cytokine response of gingival fibroblasts (GF's) from healthy and inflamed human gingival tissues and to assess whether GF's from inflamed gingivae are capable of mounting a secondary inflammatory response after exposure to P. gingivalisLPS. MATERIALS AND METHODS: GF's were obtained from healthy donors and periodontitispatients and cultured in vitro. Cells were exposed to P. gingivalisLPS for 24h before measurement of MCP-1, GRO, IL-6, IL-8 and VEGF using a bead-based multiplex assay. Statistical comparisons were made between LPS-exposed GF's and unstimulated cells as well as the two patient groups by two-way ANOVA. RESULTS: GF's exposed to P. gingivalisLPS significantly increased their production of MCP-1, GRO, IL-6, IL-8 and VEGF compared to unstimulated cells. GF's isolated from inflamed tissue from periodontitispatients demonstrated consistently less cytokine production after exposure to P. gingivalisLPS, most notably for GRO and IL-6. CONCLUSIONS: The current study demonstrates that GF's play an active role in the inflammatory response in periodontal disease by producing a number of chemokines and cytokines. Furthermore, inflamed GF's may be compromised in their ability to mount an adequate secondary immune response in relation to chemokine/cytokine production. CLINICAL RELEVANCE: The compromised inflammatory cytokine response of inflamed human gingival fibroblasts to P. gingivalisLPS may impact on their ability to recruit and activate inflammatory cells while maintaining persistent inflammation, a key feature of periodontal disease.
Authors: Ana Carolina F Morandini; Carla Renata Sipert; Thaís Helena Gasparoto; Sebastião Luiz A Greghi; Euloir Passanezi; Maria Lucia R Rezende; Adriana P Sant'ana; Ana Paula Campanelli; Gustavo P Garlet; Carlos F Santos Journal: J Periodontol Date: 2010-02 Impact factor: 6.993
Authors: Jan Korbecki; Iwona Szatkowska; Patrycja Kupnicka; Wojciech Żwierełło; Katarzyna Barczak; Iwona Poziomkowska-Gęsicka; Jerzy Wójcik; Dariusz Chlubek; Irena Baranowska-Bosiacka Journal: Int J Mol Sci Date: 2022-06-28 Impact factor: 6.208