| Literature DB >> 28688900 |
Huaping Zhang1, Zhenxiang Zhao2, Xuefen Pang3, Jian Yang2, Haixia Yu2, Yinhong Zhang4, Hui Zhou2, Jiahui Zhao2.
Abstract
The antioxidant activity of genistein is associated with preventing atherosclerosis; however, the underlying mechanisms are not fully understood. In this study, human umbilical vein endothelial cells (HUVECs) were pretreated with genistein at different concentrations (10nM, 100nM and 1000nM) for 6h and then exposed to ox-LDL (50mg/L) for another 24h. Results showed that genistein restrained reactive oxygen species (ROS) and malondialdehyde (MDA) production, and ameliorated the inhibitory effect on superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GPx) activity elicited by ox-LDL stimulation. The effects of genistein were correlated with the upregulation of sirtuin-1 via inhibiting miR-34a, and were abolished by sirtuin-1 siRNA or miR-34a mimic. Moreover, the antioxidation of genistein was associated with miR-34a/sirtuin-1-mediated nuclear translocation and deacetylation of foxo3a, accompanying with the enhanced expressions of MnSOD and CAT. The present study suggests that miR-34a/sirtuin-1/foxo3a might play an important role in genistein reversing ox-LDL-induced oxidative damage in HUVECs.Entities:
Keywords: Atherosclerosis; Genistein; Oxidative damage; Sirtuin-1; foxo3a; miR-34a
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Year: 2017 PMID： 28688900 DOI： 10.1016/j.toxlet.2017.07.216
Source DB: PubMed Journal: Toxicol Lett ISSN： 0378-4274 Impact factor: 4.372