Chen Zhong1, Zhang Mei2, Xu Yong1. 1. Dept. of Stomatology, Xiamen Medical College, Xiamen 361008, China. 2. Dept. of Stomatology, Gaoqing People's Hospital in Shandong Province, Gaoqing 256300, China.
Abstract
OBJECTIVE: The main goal is to investigate the role of P120-catenin (P120ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells. METHODS: The plasmid pGFP-V-RS-P120ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120ctn in these cells. Real-time fluorescent quantitative polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection. RESULTS: After the plasmid pGFP-V-RS-P120ctn shRNA was transfected into the TSCCA cells, we found that as the P120ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased significantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05). CONCLUSIONS: In OSCC cells, P120ctn may be involved in cadherin switching and promote metastasis and invasion.
OBJECTIVE: The main goal is to investigate the role of P120-catenin (P120ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells. METHODS: The plasmid pGFP-V-RS-P120ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120ctn in these cells. Real-time fluorescent quantitative polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection. RESULTS: After the plasmid pGFP-V-RS-P120ctn shRNA was transfected into the TSCCA cells, we found that as the P120ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased significantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05). CONCLUSIONS: In OSCC cells, P120ctn may be involved in cadherin switching and promote metastasis and invasion.
Entities:
Keywords:
P120-catenin; cadherin switching; oral squamous cell cancer
Authors: M Maeda; E Johnson; S H Mandal; K R Lawson; S A Keim; R A Svoboda; S Caplan; J K Wahl; M J Wheelock; K R Johnson Journal: Oncogene Date: 2006-06-19 Impact factor: 9.867
Authors: J Lade-Keller; R Riber-Hansen; P Guldberg; H Schmidt; S J Hamilton-Dutoit; T Steiniche Journal: Br J Dermatol Date: 2013-09 Impact factor: 9.302