Martha Patricia Gallegos-Arreola1, María Guadalupe Márquez-Rosales2,3, José Sánchez-Corona3, Luis Eduardo Figuera4, Guillermo Zúñiga-González3, Ana María Puebla-Pérez5, Jorge Iván Delgado-Saucedo5, Héctor Montoya-Fuentes3. 1. Genetics Division, Western Biomedical Research Center, Western National Medical Center, University of Guadalajara, Jalisco, México marthapatriciagallegos08@gmail.com. 2. Doctorate Program in Human Genetics, Health Sciences University Center, University of Guadalajara, Jalisco, México. 3. Molecular Medicine Division, Western Biomedical Research Center, Western National Medical Center, Mexican Institute of Social Security, University of Guadalajara, Guadalajara, Jalisco, México. 4. Genetics Division, Western Biomedical Research Center, Western National Medical Center, University of Guadalajara, Jalisco, México. 5. Immunopharmacology Laboratory, Exact and Engineering Sciences University Center, University of Guadalajara, Guadalajara, Jalisco, México.
Abstract
INTRODUCTION: The MDM2 gene plays an important role in tumorigenesis. The data on the Del1518 promoter polymorphism in the MDM2 gene have revealed associations with cancer. MATERIAL AND METHODS: We examined the role of the MDM2 Del1518 polymorphism through a comparison of the genotypes of 345 healthy Mexican women with those of 742 Mexican women with breast cancer (BC). RESULTS: The genotype frequencies of the MDM2 Del1518 polymorphism in controls and patients with BC were 64% and 55.5% for ins/ins, 32% and 31.5% for ins/del, and 4% and 13% for del/del, respectively. The obtained odds ratio (OR) was 3.26, with a 95% confidence interval (95% CI) of 1.86-5.72 and p=0.0001, for the del/del genotype. An association was evident when we examined the distribution of the del/del genotype in patients with elevated levels of transaminase SGPT (OR=2.268; 95% CI=1.40-3.65; p=0.0001). Additionally, we observed an association of the genotypes del/del - ins/del in menopausal patients with BC with the following characteristics: tobacco consumption (OR = 1.93, 95% CI = 1.07-3.4, p=0.025), pregnancy loss (OR=2.44, 95% CI=1.37-4.35, p=0.0024), obesity (I-IV) (OR=1.8, 95% CI=1.1-2.9, p= 0.018), and elevated serum glucose levels (OR=3.72, 95% CI=2.0-6.85, p=0.0001). CONCLUSIONS: The MDM2 Del1518 polymorphism was associated with BC susceptibility, particularly in menopausal patients with BC who reported tobacco consumption, pregnancy loss, obesity and high glucose levels in the analyzed Mexican population.
INTRODUCTION: The MDM2 gene plays an important role in tumorigenesis. The data on the Del1518 promoter polymorphism in the MDM2 gene have revealed associations with cancer. MATERIAL AND METHODS: We examined the role of the MDM2 Del1518 polymorphism through a comparison of the genotypes of 345 healthy Mexican women with those of 742 Mexican women with breast cancer (BC). RESULTS: The genotype frequencies of the MDM2 Del1518 polymorphism in controls and patients with BC were 64% and 55.5% for ins/ins, 32% and 31.5% for ins/del, and 4% and 13% for del/del, respectively. The obtained odds ratio (OR) was 3.26, with a 95% confidence interval (95% CI) of 1.86-5.72 and p=0.0001, for the del/del genotype. An association was evident when we examined the distribution of the del/del genotype in patients with elevated levels of transaminase SGPT (OR=2.268; 95% CI=1.40-3.65; p=0.0001). Additionally, we observed an association of the genotypes del/del - ins/del in menopausal patients with BC with the following characteristics: tobacco consumption (OR = 1.93, 95% CI = 1.07-3.4, p=0.025), pregnancy loss (OR=2.44, 95% CI=1.37-4.35, p=0.0024), obesity (I-IV) (OR=1.8, 95% CI=1.1-2.9, p= 0.018), and elevated serum glucose levels (OR=3.72, 95% CI=2.0-6.85, p=0.0001). CONCLUSIONS: The MDM2 Del1518 polymorphism was associated with BC susceptibility, particularly in menopausal patients with BC who reported tobacco consumption, pregnancy loss, obesity and high glucose levels in the analyzed Mexican population.
Authors: Martha Patricia Gallegos-Arreola; Guillermo M Zúñiga-González; Luis E Figuera; Ana María Puebla-Pérez; María Guadalupe Márquez-Rosales; Belinda Claudia Gómez-Meda; Mónica Alejandra Rosales-Reynoso Journal: PeerJ Date: 2022-05-10 Impact factor: 3.061
Authors: Giovanna C Cavalcante; Marcos At Amador; André M Ribeiro Dos Santos; Darlen C Carvalho; Roberta B Andrade; Esdras Eb Pereira; Marianne R Fernandes; Danielle F Costa; Ney Pc Santos; Paulo P Assumpção; Ândrea Ribeiro Dos Santos; Sidney Santos Journal: World J Gastroenterol Date: 2017-12-28 Impact factor: 5.742