Yi-Wen Hung1, Chia-Wen Tsai2,3, Cheng-Nan Wu2, Liang-Chun Shih4,5, Yen-Yu Chen3, Yen-Fang Liu3, Huey-Shan Hung4, Ming-Yi Shen4, Wen-Shin Chang3, DA-Tian Bau6,4,7. 1. Department of Medicine Research, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. 2. Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. 3. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. 4. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. 5. Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C. 6. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com. 7. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Metalloproteinases (MMPs) are a family of multifunctional proteins reported to be overexpressed in several types of cancers. However, the contribution of MMP8 genotype to oral cancer has not been elucidated. In this study, we focused on the contribution of polymorphisms in the promoter region of MMP-8 (C-799T) and two non-synonymous polymorphisms (Val436Ala and Lys460Thr) to Taiwanese oral cancer. MATERIALS AND METHODS: In this case-control study, MMP-8 genotype, was examined among 788 patients with oral cancer and 956 gender- and age-matched healthy controls regarding its potential to determine oral cancer risk. RESULTS: The distributions of MMP-8 C-799T, Val436Ala and Lys460Thr genotypes were not different between the oral cancer and non-cancer control groups. We also analyzed the allelic frequency distributions and no significant difference was found. As for gene-environment interaction analysis, there was an increased risk for smokers, alcohol drinkers or betel quid chewers with variant MMP-8 C-799T or Val436Ala genotypes. CONCLUSION: Our findings suggest that the polymorphisms at MMP-8 C-799T or Val436Ala may not play a major role in mediating personal risk of oral cancer; however, the detailed mechanisms require further investigation. Copyright
BACKGROUND/AIM: Metalloproteinases (MMPs) are a family of multifunctional proteins reported to be overexpressed in several types of cancers. However, the contribution of MMP8 genotype to oral cancer has not been elucidated. In this study, we focused on the contribution of polymorphisms in the promoter region of MMP-8 (C-799T) and two non-synonymous polymorphisms (Val436Ala and Lys460Thr) to Taiwanese oral cancer. MATERIALS AND METHODS: In this case-control study, MMP-8 genotype, was examined among 788 patients with oral cancer and 956 gender- and age-matched healthy controls regarding its potential to determine oral cancer risk. RESULTS: The distributions of MMP-8C-799T, Val436Ala and Lys460Thr genotypes were not different between the oral cancer and non-cancer control groups. We also analyzed the allelic frequency distributions and no significant difference was found. As for gene-environment interaction analysis, there was an increased risk for smokers, alcohol drinkers or betel quid chewers with variant MMP-8C-799T or Val436Ala genotypes. CONCLUSION: Our findings suggest that the polymorphisms at MMP-8C-799T or Val436Ala may not play a major role in mediating personal risk of oral cancer; however, the detailed mechanisms require further investigation. Copyright
Authors: Ragini D Singh; N Haridas; Jayendra B Patel; Franky D Shah; Shilin N Shukla; Pankaj M Shah; Prabhudas S Patel Journal: Indian J Clin Biochem Date: 2010-08-25