Cheng-Hsi Liao1,2,3, Wen-Shin Chang4, Chia-Wen Tsai4, Pei-Shin Hu5, Hsi-Chin Wu4, Shih-Wei Hsu1,2,3, Guan-Liang Chen1,3, Te-Cheng Yueh1,2,3, Te-Chun Shen4, Te-Chun Hsia4, DA-Tian Bau6,4,7. 1. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. 2. Department of Surgery, Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C. 3. National Defense Medical Center, Taipei, Taiwan, R.O.C. 4. Terry Fox Cancer Research Laboratory, Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan, R.O.C. 5. Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C. 6. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com. 7. Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: The breakage of matrix metalloproteinases (MMPs) has been reported to be one of the mechanisms required for tumor invasion, and the expression of MMP-7 in serum is correlated with poor prognosis of urinary bladder cancer patients. However, the role of the MMP-7 genotypes has been seldom examined among bladder cancer patients. Therefore, this study aimed at examining the promoter polymorphic MMP-7 genotypes A-181G and C-153T among Taiwanese bladder cancer patients and evaluate the contribution of the genotypic variants of MMP-7 to bladder cancer risk in Taiwan. MATERIALS AND METHODS: Three hundred and seventy-five bladder cancer patients and the same number of gender- and age-matched healthy controls were genotyped for A-181G and C-153T in the promoter of MMP-7 via polymerase chain reaction-restriction fragment length polymorphism methodology. RESULTS: The frequencies of AA, AG and GG at A-181G of the promoter of MMP-7 were 89.1, 8.8 and 2.1% in the bladder cancer patient group and 87.5, 10.9 and 1.6% in the matched healthy control group, respectively (p for trend=0.5475). There was no polymorphic genotype for MMP-7 C-153T among the Taiwanese population. The comparisons in allelic frequency distribution also support the findings that the G allele may not be the determinant allele for bladder cancer in Taiwan. In addition, the results showed that there is no significant association of the bladder risk with the MMP-7 A-181G genotype, even after adjustment for the possible confounding factors. Furthermore, there is no interaction of the genotypes of MMP-7 with age, gender, smoking and alcohol consumption on bladder cancer risk. CONCLUSION: The results of this study suggest that the two MMP-7 polymorphisms, - A-181G and C-153T, do not play a major role in determining personal susceptibility to bladder cancer in Taiwan. Copyright
BACKGROUND/AIM: The breakage of matrix metalloproteinases (MMPs) has been reported to be one of the mechanisms required for tumor invasion, and the expression of MMP-7 in serum is correlated with poor prognosis of urinary bladder cancerpatients. However, the role of the MMP-7 genotypes has been seldom examined among bladder cancerpatients. Therefore, this study aimed at examining the promoter polymorphic MMP-7 genotypes A-181G and C-153T among Taiwanese bladder cancerpatients and evaluate the contribution of the genotypic variants of MMP-7 to bladder cancer risk in Taiwan. MATERIALS AND METHODS: Three hundred and seventy-five bladder cancerpatients and the same number of gender- and age-matched healthy controls were genotyped for A-181G and C-153T in the promoter of MMP-7 via polymerase chain reaction-restriction fragment length polymorphism methodology. RESULTS: The frequencies of AA, AG and GG at A-181G of the promoter of MMP-7 were 89.1, 8.8 and 2.1% in the bladder cancerpatient group and 87.5, 10.9 and 1.6% in the matched healthy control group, respectively (p for trend=0.5475). There was no polymorphic genotype for MMP-7C-153T among the Taiwanese population. The comparisons in allelic frequency distribution also support the findings that the G allele may not be the determinant allele for bladder cancer in Taiwan. In addition, the results showed that there is no significant association of the bladder risk with the MMP-7A-181G genotype, even after adjustment for the possible confounding factors. Furthermore, there is no interaction of the genotypes of MMP-7 with age, gender, smoking and alcohol consumption on bladder cancer risk. CONCLUSION: The results of this study suggest that the two MMP-7 polymorphisms, - A-181G and C-153T, do not play a major role in determining personal susceptibility to bladder cancer in Taiwan. Copyright
Authors: A Lekstan; P Lampe; J Lewin-Kowalik; M Olakowski; B Jablonska; K Labuzek; H Jedrzejowska-Szypulka; E Olakowska; D Gorka; I Filip; D Dranka-Bojarowska Journal: J Physiol Pharmacol Date: 2012-12 Impact factor: 3.011
Authors: Edyta Wieczorek; Edyta Reszka; Wojciech Wasowicz; Adam Grzegorczyk; Tomasz Konecki; Marek Sosnowski; Zbigniew Jablonowski Journal: Cent European J Urol Date: 2014-01-27