Cristina de Mattos Pimenta Vidal1, Ariene Arcas Leme-Kraus2, Momina Rahman2, Ana Paula Farina3, Ana K Bedran-Russo4. 1. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL, 60612, USA; Department of Operative Dentistry, College of Dentistry, University of Iowa, 801 Newton Rd, Iowa City, IA, 52242, USA. 2. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL, 60612, USA. 3. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL, 60612, USA; School of Dentistry, University of Passo Fundo, BR 285, São José,Building A7, Passo Fundo, RS, 99052-900, Brazil. 4. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL, 60612, USA. Electronic address: bedran@uic.edu.
Abstract
OBJECTIVE: Proteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen. METHODS: Selective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α=0.05). RESULTS: Transmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p=0.0224) and control (p=0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix. CONCLUSIONS: GAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability.
OBJECTIVE: Proteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen. METHODS: Selective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α=0.05). RESULTS: Transmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p=0.0224) and control (p=0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix. CONCLUSIONS: GAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability.
Authors: Yvette Alania; Livia T Trevelin; Mohammad Hussain; Camila A Zamperini; Gresa Mustafa; Ana K Bedran-Russo Journal: J Mech Behav Biomed Mater Date: 2019-12-09
Authors: António H S Delgado; Madalena Belmar Da Costa; Mário Cruz Polido; Ana Mano Azul; Salvatore Sauro Journal: Sci Rep Date: 2022-07-29 Impact factor: 4.996