Hsiu-Jung Lo1, Sheng-Hua Tsai2, Wen-Li Chu3, Yin-Zhi Chen3, Zi-Li Zhou4, Hong-Fu Chen5, Ching-Fu Lee5, Yun-Liang Yang6. 1. Taiwan Mycology Reference Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan; School of Dentistry, China Medical University, Taichung, Taiwan. 2. Taiwan Mycology Reference Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan; Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan. 3. Taiwan Mycology Reference Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan. 4. Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan. 5. Department of Applied Science, Nanda Campus, National Tsing Hua University, Hsinchu, Taiwan. 6. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan. Electronic address: yyang@mail.nctu.edu.tw.
Abstract
OBJECTIVE: We investigated the diversity and drug susceptibility of pathogenic yeasts on fruit surfaces. METHOD: Fruits were purchased from supermarkets and washed with buffer. The pellets were re-suspended in medium after centrifugation. The cell suspensions were plated onto CHROMagar Candida medium. Yeasts were identified by ribosomal DNA sequencing and their drug susceptibilities were determined by broth microdilution assay. RESULTS: Of 184 isolates, comprised of 55 species, from 22 different types of fruits, 29 species, including Candida famata, Candida fermentati, Candida guilliermondii, Candida intermedia, Candida krusei, Candida orthopsilosis, Candida parapsilosis, Candida pelliculosa, Candida tropicalis, and others have been reported to cause diseases in humans. In addition to C. krusei, intrinsically resistant to fluconazole, all Rhodotorula and Rhodosporidium species were resistant to fluconazole. One each of C. tropicalis isolate was belonged to diploid sequence type (DST)149 and DST225, genotypes also detected in isolates from humans. Furthermore, the DST225 isolate was less susceptible to azole drugs. The susceptibilities to azole drugs for clinical and agricultural usage were associated to each other. CONCLUSION: It is important to be aware of the existence of pathogenic yeasts, especially drug-resistant ones, on the fruit surfaces, a potential route for pathogenic yeasts to be transmitted to humans.
OBJECTIVE: We investigated the diversity and drug susceptibility of pathogenic yeasts on fruit surfaces. METHOD: Fruits were purchased from supermarkets and washed with buffer. The pellets were re-suspended in medium after centrifugation. The cell suspensions were plated onto CHROMagar Candida medium. Yeasts were identified by ribosomal DNA sequencing and their drug susceptibilities were determined by broth microdilution assay. RESULTS: Of 184 isolates, comprised of 55 species, from 22 different types of fruits, 29 species, including Candida famata, Candida fermentati, Candida guilliermondii, Candida intermedia, Candida krusei, Candida orthopsilosis, Candida parapsilosis, Candida pelliculosa, Candida tropicalis, and others have been reported to cause diseases in humans. In addition to C. krusei, intrinsically resistant to fluconazole, all Rhodotorula and Rhodosporidium species were resistant to fluconazole. One each of C. tropicalis isolate was belonged to diploid sequence type (DST)149 and DST225, genotypes also detected in isolates from humans. Furthermore, the DST225 isolate was less susceptible to azole drugs. The susceptibilities to azole drugs for clinical and agricultural usage were associated to each other. CONCLUSION: It is important to be aware of the existence of pathogenic yeasts, especially drug-resistant ones, on the fruit surfaces, a potential route for pathogenic yeasts to be transmitted to humans.
Authors: Caoimhe E O'Brien; João Oliveira-Pacheco; Eoin Ó Cinnéide; Max A B Haase; Chris Todd Hittinger; Thomas R Rogers; Oscar Zaragoza; Ursula Bond; Geraldine Butler Journal: PLoS Pathog Date: 2021-03-31 Impact factor: 6.823
Authors: Caitlin Keighley; Mailie Gall; Sebastiaan J van Hal; Catriona L Halliday; Louis Yi Ann Chai; Kean Lee Chew; Chayanika Biswas; Monica A Slavin; Wieland Meyer; Vitali Sintchenko; Sharon C A Chen Journal: J Fungi (Basel) Date: 2022-08-23