Naomi Inoue1, Rosmary Lopez2, Andrea Delgado3, Denisse Nuñez1, Jimmy Portella1, Luis Noriega-Hoces1,3, Luis Guzmán4,5. 1. PRANOR. Grupo de Reproducción Asistida, Calle los Olivos 364, San Isidro, Lima, Peru. 2. Reprogenetics Latinoamerica, Lima, Peru. 3. Clínica Concebir, Calle Los Olivos 364 San Isidro, Lima, Peru. 4. PRANOR. Grupo de Reproducción Asistida, Calle los Olivos 364, San Isidro, Lima, Peru. lguzmanm@gmail.com. 5. Reprogenetics Latinoamerica, Lima, Peru. lguzmanm@gmail.com.
Abstract
PURPOSES: The purpose of this study is to describe a healthy life birth after a mosaic embryo transfer in oocyte in vitro maturation (IVM). METHODS: Patient received minimal stimulation, starting on day 3 after menstrual period. No hCG trigger was administered. Oocyte retrieval was performed and oocytes were matured for 30 h. After denuding, mature oocytes were inseminated by ICSI. Embryos were cultured until blastocyst stage and biopsied. RESULTS: One euploid embryo after array comprehensive genome hybridization (aCGH) was diagnostic. However, the next-generation sequencing (NGS) re-analysis showed that embryo was a mosaic for chromosome 13 and 21. Nevertheless, pregnancy ultrasound scans and non-invasive prenatal test (NIPT-Verifi-Illumina) indicated a normal fetus development. Finally, a healthy baby was born after 38 weeks. Its weight was 4480 g, head circumference 36 cm, and total length of 51 cm. To confirm that the baby was chromosomically normal, an NGS test was performed in buccal cells, a normal profile was obtained. CONCLUSIONS: Our finding confirmed that mosaic embryo transfer would bring a healthy offspring.
PURPOSES: The purpose of this study is to describe a healthy life birth after a mosaic embryo transfer in oocyte in vitro maturation (IVM). METHODS:Patient received minimal stimulation, starting on day 3 after menstrual period. No hCG trigger was administered. Oocyte retrieval was performed and oocytes were matured for 30 h. After denuding, mature oocytes were inseminated by ICSI. Embryos were cultured until blastocyst stage and biopsied. RESULTS: One euploid embryo after array comprehensive genome hybridization (aCGH) was diagnostic. However, the next-generation sequencing (NGS) re-analysis showed that embryo was a mosaic for chromosome 13 and 21. Nevertheless, pregnancy ultrasound scans and non-invasive prenatal test (NIPT-Verifi-Illumina) indicated a normal fetus development. Finally, a healthy baby was born after 38 weeks. Its weight was 4480 g, head circumference 36 cm, and total length of 51 cm. To confirm that the baby was chromosomically normal, an NGS test was performed in buccal cells, a normal profile was obtained. CONCLUSIONS: Our finding confirmed that mosaic embryo transfer would bring a healthy offspring.
Authors: A Mertzanidou; L Wilton; J Cheng; C Spits; E Vanneste; Y Moreau; J R Vermeesch; K Sermon Journal: Hum Reprod Date: 2012-10-09 Impact factor: 6.918
Authors: C Spits; L Guzman; A Mertzanidou; K Jacobs; C Ortega-Hrepich; R B Gilchrist; J G Thompson; M De Vos; J Smitz; K Sermon Journal: Hum Reprod Date: 2014-12-04 Impact factor: 6.918
Authors: R Fadini; M B Dal Canto; M Mignini Renzini; F Brambillasca; R Comi; D Fumagalli; M Lain; M Merola; R Milani; E De Ponti Journal: Reprod Biomed Online Date: 2009-09 Impact factor: 3.828