Bahareh Sadat Rasouli1, Ali Ghadimi-Darsajini1, Reza Nekouian1, Gholam-Reza Iragian2. 1. Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. 2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Gastric cancer (GC) is the third leading cause of cancer death, and most patients represent metastatic phenotype at the time of diagnosis. Urokinase plasminogen activator (uPA) system is well known for its critical roles in cancer cells invasion since uPA/uPA receptor (uPAR) overexpresses in several cancers. Subsequently, suppression of uPA/uPAR gene expression improves patients overall survival and prevents cancer progression. OBJECTIVES: The aim of the current study was to investigate possible effects of live Lactobacillus reuteri as a probiotic in inhibition of GC cells proliferation and invasion. MATERIALS AND METHODS: Human gastric adenocarcinoma epithelial cell line (AGS) cells were treated with different ratios of live L. reuteri and were incubated for 24, 48, and 72 h. Viability of cancer cells was measured with 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay, and the effects of L. reuteri on uPA/uPAR gene expression were assessed by real-time polymerase chain reaction. RESULTS: Our results showed that L. reuteri inhibits cell proliferation significantly in dose-dependent manner. Expressions of uPA and uPAR were downregulated followed by co-incubation of AGS cells and live L. reuteri compared to untreated-based line level. CONCLUSION: This study provides strong support in the role of L. reuteri in suppression of GC cell invasion by downregulation of pathways which is involved in extracellular matrix degradation such as uPA and uPAR.
BACKGROUND:Gastric cancer (GC) is the third leading cause of cancer death, and most patients represent metastatic phenotype at the time of diagnosis. Urokinase plasminogen activator (uPA) system is well known for its critical roles in cancer cells invasion since uPA/uPA receptor (uPAR) overexpresses in several cancers. Subsequently, suppression of uPA/uPAR gene expression improves patients overall survival and prevents cancer progression. OBJECTIVES: The aim of the current study was to investigate possible effects of live Lactobacillus reuteri as a probiotic in inhibition of GC cells proliferation and invasion. MATERIALS AND METHODS:Humangastric adenocarcinoma epithelial cell line (AGS) cells were treated with different ratios of live L. reuteri and were incubated for 24, 48, and 72 h. Viability of cancer cells was measured with 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay, and the effects of L. reuteri on uPA/uPAR gene expression were assessed by real-time polymerase chain reaction. RESULTS: Our results showed that L. reuteri inhibits cell proliferation significantly in dose-dependent manner. Expressions of uPA and uPAR were downregulated followed by co-incubation of AGS cells and live L. reuteri compared to untreated-based line level. CONCLUSION: This study provides strong support in the role of L. reuteri in suppression of GC cell invasion by downregulation of pathways which is involved in extracellular matrix degradation such as uPA and uPAR.
Authors: Ahmad Javanmard; Sara Ashtari; Babak Sabet; Seyed Hossein Davoodi; Mohammad Rostami-Nejad; Mohammad Esmaeil Akbari; Azadeh Niaz; Amir Mohammad Mortazavian Journal: Gastroenterol Hepatol Bed Bench Date: 2018
Authors: Casey T Finnicum; Zahraa Rahal; Maya Hassane; Warapen Treekitkarnmongkol; Ansam Sinjab; Rhiannon Morris; Yuejiang Liu; Elizabeth L Tang; Sarah Viet; Jason L Petersen; Philip L Lorenzi; Lin Tan; Joseph Petrosino; Kristi L Hoffman; Junya Fujimoto; Seyed Javad Moghaddam; Humam Kadara Journal: Int J Mol Sci Date: 2022-09-18 Impact factor: 6.208
Authors: Alastair M McKee; Benjamin M Kirkup; Matthew Madgwick; Wesley J Fowler; Christopher A Price; Sally A Dreger; Rebecca Ansorge; Kate A Makin; Shabhonam Caim; Gwenaelle Le Gall; Jack Paveley; Charlotte Leclaire; Matthew Dalby; Cristina Alcon-Giner; Anna Andrusaite; Tzu-Yu Feng; Martina Di Modica; Tiziana Triulzi; Elda Tagliabue; Simon W F Milling; Katherine N Weilbaecher; Melanie R Rutkowski; Tamás Korcsmáros; Lindsay J Hall; Stephen D Robinson Journal: iScience Date: 2021-08-20